Chromosome number was investigated in a number of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and different stages of meiosis. All stevia strains were found to had 2n = 22. The number of bivalents at diakinesis was 11 for all the studied strains. Analysis of SDS-PAGE of soluble protein revealed complete similarity (100%) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band. Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR). A total of 231 clear bands were generated, out of which 34 (14.72 %) were unique bands. The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer.
Bekhit, M. (2018). Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in Stevia rebaudiana (Bertoni).. Annals of Agricultural Science, Moshtohor, 56(4th ICBAA), 177-186. doi: 10.21608/assjm.2018.65136
MLA
Makhlouf Bekhit. "Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in Stevia rebaudiana (Bertoni).". Annals of Agricultural Science, Moshtohor, 56, 4th ICBAA, 2018, 177-186. doi: 10.21608/assjm.2018.65136
HARVARD
Bekhit, M. (2018). 'Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in Stevia rebaudiana (Bertoni).', Annals of Agricultural Science, Moshtohor, 56(4th ICBAA), pp. 177-186. doi: 10.21608/assjm.2018.65136
VANCOUVER
Bekhit, M. Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in Stevia rebaudiana (Bertoni).. Annals of Agricultural Science, Moshtohor, 2018; 56(4th ICBAA): 177-186. doi: 10.21608/assjm.2018.65136
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