Effect of Wheat Germ oil and Coenzyme Q10 on Physiological Performance and Testicular Oxidative Stress Markers in Rabbit Bucks

The aim of this study is to investigate the efficacy of WGO and Coenzyme Q10 low and high doses on physiological performance and testicular oxidative status of male rabbit bucks. In addition tracing the best suitable interval which modulates physiological response under Egyptian summer condition. This experiment was done on a pure strain of Sinai gabali bucks 3 months old. The animals were randomly divided into four groups each one comprise 6 animals. The 1^st group served as normal control (C), the 2^nd group treated with wheat germ oil (WGO) (300 mg/kg BW), the 3^rd group treated with Coenzyme Q10L (10 mg /kg BW), and the 4^th group treated with Coenzyme Q10H (20 mg/kg BW).Rabbits of all treatments were given oral administration daily for 60 days, and blood samples were collected monthly along 6 months after the end of the treatment. Moreover, the rabbits were decapitated and the testes tissues were exceed for evaluation Reduced Glutathione (GSH), Malondialdehyde (MDA), Oxidized Glutathione (GSSG) and Nitric oxide (NO) contents. Data revealed that liver function did not show any significant differences due to treatment. Month effect did not show any differences in ALT and AST values. According to the interaction of treatment and month data showed the best value of ALT and AST (26.48 and 32.72 U/L, respectively) in C× 6^th and Coenzyme Q10L ×5^th month, respectively. With regard to Lipid profile data showed significant decrease (P<0.05) of total cholesterol, LDL and triglyceride due to Coenzyme Q10L treatment and significant (P<0.05) increase in HDL due to Coenzyme Q10L treatment and the interaction showed the best values for TC, TG, HDL and LDL (89.50 mg/ dL Coenzyme Q10L×2^nd month, 87.50 mg/ dL Coenzyme Q10L×3^rd month, 36.00 mg/ dL Coenzyme Q10L×3^rdmonth and 35.96 mg/ dL Coenzyme Q10L×3^rdmonth, respectively). Results did not show any significant (P˃ 0.05) differences in blood proteins in the 4 groups due to the effect of treatments on male rabbits. Also month effect data did not show any changes (P˃ 0.05) in blood proteins. The interaction showed the best values of blood proteins, total protein ,albumin ,globulin and A/G ratio, 7.63g/dL in Coenzyme Q10H×4^th month (hot THI) , 4.17g/dL in WGO ×2^nd month (hot THI), 3.58g/dL in Coenzyme Q10H ×4^th month(hot THI) and 1.30 in Coenzyme Q10H ×6^th month (mild THI), respectively). Oxidative markers of testicular MDA and GSH showed decreases in MDA and increase in GSH due to WGO and Coenzyme Q10 treatments, respectively. In conclusion, the present study suggests that Coenzyme Q10 and WGO treatments improve physiological performance, reduced heat stress and testicular oxidative stress markers in Sinai gabali male rabbits under Egyptian summer condition.