Characterization of Bioactive Components of Callistemon Lanculatues by HPLC-DAD.

Document Type : Original Article

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Agriculture Biochemistry Department, Faculty of Agriculture, Benha University, Egypt

Abstract

Callistemon lanculatues leaves and flowers contain active components. So this could be used as an antimicrobial and antioxidant component. This study aims to determine the chemical composition, and the best method for extracting total phenolic, total flavonoids, and antioxidant activity by DPPH to identify the most important uses of this plant. Six solvents were used according to differences in polarity: water, methanol, ethanol, ethyl acetate, chloroform, and petroleum ether respectively. Methanol extract showed the highest activity as antimicrobial, so it identified the active components of this extract by HPLC-DAD analysis. Results showed that the highest concentration of antioxidant activity in methanol extract was 40.9 %, while ethanol extract was 38.7% in the leaves. In flowers, methanol extract activity was 33.97%, while ethanol extract was 36.1%. Nine phenolic components were identified the highest amount quinic showed (0.1682 mg/ml). Followed by cinnamic (0.0192 mg/ml) in flowers, while in leaves quinic showed (0.0529 mg/ml) and cinnamic (0.0180 mg/ml). Flavonoid Kaempferol showed the highest concentration 533.52 mg/ml) followed by (Diosmin 258.127 mg/ml) in flower, while in leaves Kampferol showed (195.73 mg/ml) and Quercitin (118.73 mg/ml).

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