Realized Heritability, Cross Resistance and Stability of Sulfoxaflor Resistance in the Cowpea Aphid, Aphis Craccivora (Koch.)

Darwish, Shereen Mohamed; Halawa, Safaa Mahmoud; Eid, Sara; Mokbel, El-sayed

doi:10.21608/assjm.2023.318491

Realized Heritability, Cross Resistance and Stability of Sulfoxaflor Resistance in the Cowpea Aphid, Aphis Craccivora (Koch.)

Document Type : Original Article

Authors

¹ Plant Protection Agricultural Benha universty

² planet protection department ,Faculty of agriculture , Benha university

³ Plant protection Agricultural Benha universty

⁴ Agricultural Research Center,central agricultural pesticide laboratory, Egypt

10.21608/assjm.2023.318491

Abstract

Aphis craccivora is a serious insect pest. attacks leguminous crops in Egypt. It causes harm through directly sapsucking or through indirectly viral diseases transmission. Aphid control mainly rely on the use of synthetic insecticides. Sulfoxaflor, a fourth-generation neonicotinoid, used to manage sap-sucking pests which had developed resistance to other insecticide. But, risk assessment of sulfoxaflor resistance in the cowpea aphid has not been studied before. So, the leaf-dip bioassay method was used to predict sulfoxaflor resistance, cross resistance and resistance stability in A. craccivora. Sulfoxaflor resistant strain of the cowpea aphid was obtained by selecting the field strain for 17 generations. The sulfoxaflor-selected strain (SFX- SEL strain) showed a 125.39-fold. Realized heritability (h²) of resistance was calculated to be 0.19. According to predicted rates of sulfoxaflor resistance indicated that, if h² = 0.19with is fifty percent at each generation, then a tenfold increase in LC₅₀ would beanticipated in 12.29 generations. The obtained results showed increased levels of cross-resistance to flupyradifurone (18.16-fold),and pymetrozine (14.19-fold). Oppositely, the R-strain did not show cross-resistance to pirimicarb (3.64-fold), carbosulfan (3.5-fold), malathion (3.41-fold), dinotefuran (3.39-fold), -cyhalothrin (1.22-fold), and fipronil (1.12-fold), respectively. Fortunately, resistance to sulfoxaflor was reversed aroundcontrol strain throughout 20 generations without exposur to any insecticide. Our findings revealed the cowpea aphid's ability to evolve sulfoxaflor resistance under continual selection pressure. The retreat of sulfoxaflor makes A. craccivora compatible with techniques for managing resistance such as non-cross-resistant insecticides rotation.

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