Effect of Different Barley Leaves Extracts On Blood Lipid Profiles in High Fat Diet of Experimental Rats

The present study aimed to investigate the phytochemicals constituent of water and ethanolic extracts of barley leaves at 15 and 20 day old sprout. Also, evaluated the protective effect of these extracts against the blood lipid profile levels  in high fat diet (HFD) of experimental rats. Finally, histopathological evaluation of liver and kidney were performed. The phenolic compounds content in barley leaves aged 15^th day of germinated were found to be 5425.4 and 7237.6 mg GAE/100 g, for both water and ethanol extracts while, in barley leaves aged 20^th day of germinated of water and ethanol extracts were 5960.4, 8792 mg GAE/100 g. However, total flavonoids (TF) of both water and ethanol extracts of barley leaves aged 15^th and 20^th day of germinated were found to be (83.82 and 145.2 mg QE/100 g) and (167.9 and 191.8 mg QE/100 g) of DW, respectively. While, the antioxidant activity in the germinated barley for both water and ethanol extracts aged15^th day showed 55.68 and 67.16% for (DPPH) and were found to be 77.68, 42.21% for (ABTS). However, barley leaves both water and ethanol extracts aged 20^th day of germinated were 65.66 and 68.84% for (DPPH) and for (ABTS) were 79.33 and 52.30 %, respectively. Total carotenoids content were found to be (0.064, 0.072, 0.185 and 0.134 mg/100 g) but β-glucan contents were found to be (9.16, 5.47, 1.89 and 1.2 μg/mL) for water and ethanol extracts of barley leaves aged 15th and 20^th day of germination, respectively . sixity rats used for Biological Experiment to study the effect of different barley leaves extracts on lipid profiles in HFD.
From the obtained results, it could be seen that the serum TG, TC, LDL-C, VLDL-C, and risk factor had a significant reduced in all HFD groups treated with different extracts of barley leaves when compared with positive control . Also, both water and ethanol extracts of barley leaves extracts were found to be improved in serum ALT and AST of experimental rats .In addition the treated and protected rats with barley leaves extract groups showed a significant decrease in urea, creatinine and uric acid levels when compared to hyperlipidemic group. Histopathological variationobserved in liver and kidney tissue of high fat groups when treated with barley leaves extracts showed improved their tissues. Finally, it could be concluded that barley green leaves extracts (ethanol and water) can be used as a lowering hyperlipidemic rats subsequently prevent from heart diseases caused by arteriosclerosis. Moreover, this extracts might be improve liver and kidney function.