DNA-barcoding and Identification for Some Common Bean and Teparry Bean Genotypes using rbcL Gene

Document Type : Original Article

Author

Faculty of Agriculture at Moshtohor, Benha University,Egypt.

Abstract

Among the different biological sources, the common bean, tepary Bean, and the wild type (Phaseolus spp < /em>.) have many biotechnological applications. The DNA barcode of the use of the rbcL gene has proven its usefulness in the study of Phaseolus spp. Phylogenetic diversity of genotypes, more than a cryptic introduction, environmental modulation, geographic distribution, and species identification in exclusive bean species. Eleven samples of common bean had been obtained from unique locations in Egypt. The gene of rbcl had proven successful in PCR reaction. Seven samples of complete array with size 4031 base pair have been obtained. The spot ranged from 574 to 575 bp with a common size of 576 bp. The identification of the species published that the samples of the specimen River Garow, Bronco, Cerdon, Goru, Giza3 three belong to Phaseolus vulgaris, and two samples of the specimen Teparry113, Teparry116 belong to Phaseolus acutifolius, respectively. The rbcL-based DNA barcode was once nearly profitable to discover distinctive specimens of Phaseolus spp < /em> according to species and genus. Some samples of the rbcL gene were no longer sufficient to perceive the stage of the genus and could not differentiate between relatively comparable species.

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Annals of Agricultural Science, Moshtohor
Volume 60, Issue 1
March 2022
Pages 119-126

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