Biological control of tomato wilt disease incited by Fusarium oxysporum f.sp lycopersici and there effects on seed germination and other biological parameters

Document Type : Original Article

Author

*Plant Pathology Dept., Fac. Agric. El-Minia Unv. Egypt.

Abstract

In previous paper it has been reported that Fusarium oxysporum f.sp < /strong> lycopersici was the incident of Fusarium wilt disease in some Egyptian governorates (Saleh et al., 2016).This paper delt with biological control of the pathogenic fungus. Trichoderma harzianum , Trichoderma viride, Bacillus subtilis and Bacillus megaterium were isolated from rhizospher of naturally wilted infect plant and identified as abovementioned . The bioagents showed strong antagonistic effect in  vitro and reduced disease severity under filed  conditions. T. harzianum proved more powerful than T. viride  in reducing disease severity while B. subtilis proved slightly more powerful than  B. megaterium under this investigation. Moreover, T. harazianum, B. subtilis and Rhizo – N were more powerful indecreasing disease severity than other tested bioagents and surpassed the  plant – guard and Moncren fungicide. Culture filtrate of Fusarium oxysporum f. sp Lycopersici reduced seed germination of tomato and affected other botanical parameters indicating that it is hazardous and notorious.

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