Biological and Molecular Characteristics of Potato Virus X Naturally Infected Potato Plants

Document Type : Original Article

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PLANT PATHOLOGY dep. Fac. of. AGR

Abstract

      Potato viruses were beenidentified in several countriesaround the world included Egypt. Potato virus X (PVX) , is one of the common   viruses   infecting  . potato crops . In the current study PVX isolated from naturally mixed viruses and identified  based on biological characters ;  serologically and molecularly. The incidence  of , PVY , PVX , PLRV, AMV, TYLCV  were found   single and mixing infection with 33.3, 37.1  , 12.38, 12.38, 5.12 %  frequency respectively out of 105 natural infected potato plants  cv. Sponta .  PVX was detected  by distinct symptoms (severe mosaic, yellow blotch ) and DAS-ELISA . It was isolated on Chenopodium.amaranticolor and Datora. stramonium L  .It had   wide host range ,  transmitted mechanically by syringe injection and Grafted tuber ,. The virus particles rod  with length wide (475) nm and (12) nm  . The virus stability was TIPexpected at 800C for 10 min. DEP expected at 10-8  and  LIVexpected at 7days. It was formed amorphous and crystal inclusion bodies in epidermal cells .  PVX have antigenicity character that serological precipitation reactionwith DAS-ELISA  . PVXinfectivityrevealed that 90% , disease severity  85%  and    high concentration in infected potato plants .The partial nucleotide sequence of  CP gene was  about 433  bp.  The genetic distance with other recommended PVX strains registered in GenBank with 10 bar  . The similarity values were recorded 100% with GU384726 1,  97.92  %  with  Accession no.MK587495 , MF662526 , KR605392 , KR605379 , KR605360  and  98.30% similarity with strain no.KJ631111..The predict numbers 144 amino acids   were starting with Tyrosine  . The genetic distance with other recommended PVX strains registered in GenBank with 0.20 bar It was recorded in gene bank with accession number MW 650651

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