Molecular Studies and Soma-Clonal Variations in Some Potato Genotypes

Document Type : Original Article

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Genetics and Genetic Engineering Dept., Faculty of Agriculture, Benha University, Egypt

Abstract

Potato is the fourth most important crop of the world wide annual production approaching 300 million tons. The tuber, the most important part of the plant, is an excellent source of complex carbohydrates, proteins, and vitamins. Salt accumulation is one of the main factors that diminish crop productivity since most of the crops are not halophytic against its toxic effects on plants also lead s to loss chloroplast activity as metabolic changes. The main objective of this study was to examine the powerful tissue culture system with three different potato genotypes. In addition to induce genetic variabilities through soma-clonal variation. Moreover, evaluate saline, and drought tolerance within the obtained clones.Results show that, The Shoot number of the three potato genotypes significantly affected by the two concentrations of sodium chloride (1000 and 2000 ppm).The shoot number of Daimont genotype was highly significant decreased in respons to the salt trearments (3.68 at 1000 ppm and 3.96 at 2000 ppm as compared with the control, 5.91). While shoot length of the three studied potato genotypes was affected by six combinations of plant hormones (auxin cytokinin) especially sponta (3.489). Trehalose,in three concentrations, exhibited an effect on shoot length of the three potato genotypes under study. Results of the molecular genetics study using IssR, showed the presence of polymorphic bands in variants and mutants suggesting that in response to all the studied treatments comparing with control.

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