Short-Term Conservation of Somatic Embryo Clusters for Date Palm (Phoenix Dactylifera l.) Cv. Amry

Document Type : Original Article

Author

Department of Horticulture , Faculty of Agriculture, Al-Azhar University, Nasr city ,Cairo, Egypt.

Abstract

This work aimed to develop a conservation protocol for date palm cv. Amry, an Egyptian semi dry rare cultivar. Slow growth conditions of storage studied by using low temperature at 15°C, beside the manipulation of salt strength of nutrient medium,( MS, 1/2 MS or1/4 MS) in combination with osmotic agent addition sucrose, sorbitol, or mannitol at constant concentration  (0.3 M) for each, to conserve somatic embryos clusters explants, which obtained previously through direct somatic embryogenesis of inflorescences explants. Survival percentage and recovery were evaluated after 6 months of conservation period. Results showed that conserved somatic embryos clusters explants on full MS, ½ MS, conservation medium supplemented with sucrose or sorbitol recorded the highest significant survival percentage on full MS,  ½ MS, where the lowest  significant  survival percentage was obtained when somatic embryos were  conserved on 1/4 medium supplemented with mannitol  . Best shoot number and shoot length recovery under normal growth conditions achieved on conservation medium supplemented with sucrose in comp inaction with reducing the full MS salt strength to the half for 6 months of conservation period under low temperature at 15°C.

Keywords

Main Subjects

Files

Share

How to cite

Statistics