Efficiency of Nanoparticles Dietary Supplement on The Growth Performance of Nile Tilapia "Oreochromis Niloticus" Intoxicated With Aflatoxin B1.

Document Type : Original Article


Department of Aquatic animal disease and management, Faculty of Vet medicine, Benha University, Egypt


The examined fish intoxicated with aflatoxin B1 (AFB1) showed several non-specific signs, including inadequate growth, significant losses in aquaculture industry especially resulting from aflatoxicosis. The study using Zinc oxide (ZnO) and Hematite (α-Fe2O3) nanoparticles (NPs) to assess the effect of using as on their own negative impacts of AFB1 3 ppm/ Kg for 13 weeks was studied 360 healthy fingerlings of Nile tilapia, were divided into eight groups (15 fish/ group) with three replicates, it has been applied in 24 aquariums. Group < sub>1 (G1) fed only a basal diet as control. While, G2, G3, and G4 were fed on diets supplemented with 2 g/kg diet of ZnO NPs, Hematite NPs and combination of both respectively. Group < sub>5 (G5) was fed on diets contamination with AFB1 (3 ppm/kg diet), while G6, G7 and G8 were fed on diets containing 2 g/ kg diet of (ZnO NPs, Hematite NPs and combination of both) plus AFB1 (3 ppm/kg diet) respectively.
Experimental treatments affected significantly on the Final Body Weight (FBW), Daily Gain (DG), Feed Conversion Ratio (FCR) and mortality Table (1, 2(. FBW and DG reduced to 37.856 g and 0.227 g in fish feed containing AFB1, when compared with groups AFB1 which supported with feed additives (ZnO, Hematite and combination of both) NPs, which reported final body weight 45.507, 47.007, 49.762 g and daily gain 0.313, 0.328, 0.360 g respectively. The highest FBW and DG were determined in group treated with feed additives combination of (ZnO and Hematite) nanoparticles, keep it track of by the fish group fed diet supplemented with Hematite NPs then those fed diet supplemented with ZnO NPs and the recorded values were for final body weight 55.857, 61.500, 63.874 g daily gain 0.427, 0.488, 0.515 g respectively than that of the control group G1 52.784, g FBW and 0.393 g DG.
The highest survival rate was obtained in fish group treated with combination of (ZnO and Hematite) nanoparticles, The survival rate was (95.556 %) in fish fed diets combination of (ZnO and Hematite) NPs, while it was 57.778 % in fish group G5 fed AFB1, which enhanced in aflatoxicated group G8 which supplemented with combination of (ZnO and Hematite) NPs 84.444 %, and in aflatoxicated group G7 which supplemented with Hematite NPs 77.778%,  and in aflatoxicated group G6 which supplemented with ZnO NPs 75.556%.
The best FCR was obtained in same order in fish group treated with combination of ZnO NPs and Hematite NPs, Hematite NPs then ZnO NPs 1.187, 1.170, 1.253 compared to the control group which recorded 1.462. On the other hand, aflatoxicated groups significantly affected feed conversion impaired significantly affected, aflatoxicated group recorded 1.950 but when supported with ZnO NPs, Hematite NPs and combination of both recorded 1.541, 1.525 and 1.502 respectively.


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