eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
1
2
10.21608/assjm.2018.57227
57227
Original Article
Super green rice in China (Abstract)
Yongzhong Xing
yzxing@mail.hzau.edu.cn
1
National Key Lab of Crop Genetic improvement, Huahzong Agricultural University, 430070, Wuhan China
Rice production in China has abused chemical fertilizers and pesticides for a long time, causing serious environmental pollution. In response to this major social issue, Chinese scientists proposed the second green revolution concept of "less investment, more output and better environment" at the end of the 20th century. In 2007, Chinese scientists proposed the concept of green super rice with the characters of "fewer pesticides, less chemical fertilizers, water saving and drought resistance, high yield and superior quality, and comfortable environment ". In 2010 and 2014, Huazhong Agricultural University as the coordinator hosted the key project of 863 Program of Ministry of Science and Technology "cultivation of new green super rice varieties". They suggested that Green super rice cultivation should divided into three phases. The first phase is to cultivate rice varieties resistant to many major diseases and pests, and thereby without spraying pesticides. The second phase is to cultivate varieties with a higher utilization efficiency and absorption efficiency of water and nitrogen and phosphorus fertilizers, thereby reducing the use of chemical fertilizers. The third phase is to cultivate a significantly enhanced drought resistance of new rice varieties, to achieve the goal of drought and water conservation through biotechnology, molecular marker-assisted selection and genome-wide selection technology. After the three-phase goal is reached, rice production will eventually achieve "less pesticides, less chemical fertilizers, water saving and drought resistance, high yield and super quality". Over the past 20 years, several reference genome sequences have been released one after another, which promotes significant progress of rice functional genomics. Special resources have been identified by establishing a rice core collection. For example, resources are resistance to pests and diseases, low nitrogen and phosphorus and drought stress, or show good performance for other target traits. Dozens of genes for grain yield, quality, plant architecture, biotic stress and abiotic stress have been isolated, which provide gene resources for the cultivation of green super rice through genome-wide selection to pyramid different good genes in the background of high-yielding varieties. More than 20 varieties (combinations) have been released at provincial or national levels. About 70 lines with green traits have been selected to adapt to different rice growing areas. Super green rice new varieties have been grown 1.4 million hectares, and reduced amounts of pesticides, fertilizers and other labor inputs, saved irrigation water, and brought significant social and economic benefits, significant ecological and environmental benefits.
https://assjm.journals.ekb.eg/article_57227_4e63406b7c6bd9572d3c5fdb68ca63aa.pdf
China
varieties of super green rice
diseases and pests rice
drought resistance
rice functional genomics
genome-wide selection
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
3
16
10.21608/assjm.2018.57243
57243
Original Article
Application for Hybrid Rice Technology at Egypt
Mahmoud Abo-Yousef
abo_yousef709@yahoo.com
1
Head of research Rice Research department- FCRI- ARC
Commercial exploitation of hybrid vigor is one of the most important applications of genetics in agriculture. It has not only contributed to food security, but has also benefited the environment, the hybrid rice Produced by crossing between two parents and the productivity increased by 20-30% than the inbred high yielding variety. The systematic of hybrid rice included three line system, two line system and One line system. The PCR technique can be used as one of the indicator for predicting the genetic purity of CMS lines, based on polymorphism among parental lines, this characteristic has been used in China to perform preliminary evaluation of the purity of hybrid rice seeds. Hybrid rice research was initiated in Egypt in 1982, to determine natural fertility restoration of Egyptian varieties on some Chinese CMS lines, evaluate certain CMS lines, test productivity of some hybrid varieties and to estimate yield and determination of seed setting percentage of some CMS lines under Egyptian conditions, but, the first Egyptian hybrid was released during 2004. For the two line system, discovery and successful utilization of environmental genic male sterility (EGMS) led to development new promising hybrids of the two lines system in hybrid rice breeding program, compared with the three-line system. EGMS trait is governed by major genes could be transferred to any genetic background and no need to use restorer genes in the male parents of two line hybrids. Finally, this system is ideal for developing indica / japonica hybrids because most japonica lines do not possess restorer genes. Molecular studies is concerning on selecting populations which can be used as the new reverse-thermo sensitive genic male sterile (rTGMS) lines to produce Japonica hybrids under Egyptian condition by using one specific SSR primer RM222.
https://assjm.journals.ekb.eg/article_57243_ac1587617eeecdcdd1b6f8468592ce65.pdf
Hybrid
rice
technology
PCR
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
17
22
10.21608/assjm.2018.57244
57244
Original Article
Genetic Fingerprinting of Root Knot Nematode as important Pest in EGYPT
SANAA HAROON
sanaaharoon1951@gmail.com
1
Fayoum University, Faculty of Agriculture, Plant Protection Department
Root knot nematode is considered as one of the most important nematode all over the world in Egypt it was found out that theses nematode attack the root system of most vegetables, fruits and Feld crops causing significance damages and economic loss .Using the morphological characterization isn't enough for nematode identification to species. So different molecular technology was used to identify different isolated of these nematode for fast and more accurate results. The internal transcript spacer (ITS) and restriction fragment length polymorphism (RFLP) of Ribosomal DNA (rDNA) sequences were used to distinguish between different major species of root knot nematode. DNA fragments containing the internal transcribed spacer (ITS) rDNA were amplified from DNA genome of different Meloidogyne spp. isolates from different governorate in Egypt. Ten females were used to amplify PCR product. When primers 5368 and 5367 were used for amplification of the ITS region, every isolate from the Meloidogyne spp. gave one major product of approximately 760 bp.
When ITS regions of all isolates were digested with restriction enzymes, by using RFLP test (Restriction fragment length polymorphisms), the size of the DNA fragment using Hind III was strong band at 560 bp and another weak band at 200 bp that is typical for M. incognita and M. javanica with Hinf I, bands of 440 and 320 pb were obtained while 520, 240 bp bands were cleared when EcoRl are used. Four bands of 220, 200, 180 and 160 bp were obtained when I restriction enzyme was used. In multiplex test, a fragment of 415 bp was obtained when M. incognita was used as template (positive control), that confirming the fact that most nematodes isolates in Egypt are M. incognita
https://assjm.journals.ekb.eg/article_57244_b9cc16abec1228cf6d1233136efbf080.pdf
Meloidogyne
multiplex
PCR
ribosomal DNA
ITS
RFLP
scar
restriction enzymes
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
23
30
10.21608/assjm.2018.57252
57252
Original Article
Genomic approaches and bioinformatics tools to identify genomic regions for economic traits and their applications in chicken breeding programs
MOSTAFA NASSAR
mostafa.nassar@agr.cu.edu.eg
1
Department of Animal Production, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt
Most of the economic traits considered in genetic improvement programs are of quantitative nature. They are genetically determined by many genes. To apply major genes or linked markers in gene- or marker-assisted selection program, they must first be identified in the genome. Mapping genomic regions for the economic traits is the first step to identify genes influencing traits of interest. This report gives the most comprehensive information on discovering quantitative traits loci (QTL) and their underlying genes for economic traits in chicken, in particular on chromosome 4 (GGA4), using up-to-date genomic approaches and bioinformatics tools. This work is based on several publications (Goraga et al. 2010, 2012; Nassar and Brockmann 2011, 2013; Nassar et al. 2012, 2013, 2015; Lyu et al. 2016, 2017) and other published QTL results in chicken QTL database (Chicken QTLdb). I had done this work in collaboration with Humboldt-Universität zu Berlin, Germany, during the year 2010 to 2017. In brief, we mapped several genomic regions on 22 chromosomes affecting 24 traits. The majority of identified loci showed additive effects on several growth and body composition traits. The biggest effect on analysed traits was detected on the distal region of GGA4. The confidence interval of the QTL region on GGA4 harbours hundreds of genes. The final identification of genes and mutations will contribute to our understanding of the complex inheritance pattern of growth regulation, muscle development and fat deposition in chicken. Such information would support breeders in using this information for genetic improvement in breeding programs.
https://assjm.journals.ekb.eg/article_57252_9b729aec50152b08d627484fdf4ce0cc.pdf
Growth
muscle mass
fat deposition
candidate gene
QTL mapping
Bioinformatics
whole genome sequencing
Microsatellite
SNP arrays
linkage and association analyses
molecular breeding
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
31
32
10.21608/assjm.2018.57254
57254
Original Article
Impact of Nanotechnology on the Future Agriculture: The Road toward Smart Agriculture (Abstract)
Taher Salah
t1salah@hotmail.com
1
Nanotechnology Research Centre, British University in Egypt
Over the past few years, scientific data indicate that nanotechnology has potential impact on the agrifood sector, minimizing adverse problems of agricultural practices on environment and human health, improving food security and productivity (as required by the predicted rise in global population), while promoting social and economic equity.
Smart agriculture (SA) is an approach that helps to guide actions needed to transform and reorient agricultural systems to effectively support development and ensure food security according to a changing climate. Nanotechnology draw the road toward the smart agriculture and aims to tackle three main objectives of SA: sustainably increasing agricultural productivity and incomes; adapting and building resilience to climate change; and reducing and/or removing greenhouse gas emissions, where possible. Approaches of Nanotechnology provide the means to help stakeholders from local to national and international levels identify agricultural strategies suitable to their local conditions.
In this context, we select and report on recent trends in nanomaterial-based systems and nanodevices that could provide benefits on the food supply chain specifically on sustainable intensification, and management of soil and waste. Among others, nanomaterials for controlled-release of nutrients, pesticides and fertilizers in crops are described as well as nanosensors for agricultural practices, food quality and safety.
https://assjm.journals.ekb.eg/article_57254_9f81a523f1f8c86d7c50977669a921e9.pdf
Nanotechnology
Agrifood
Smart Agriculture
Nanomaterials
water
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
33
34
10.21608/assjm.2018.57257
57257
Original Article
Molecular analysis of crossbreeding experiment to improve immune genetic response against salmonella in poultry (Abstract)
Maher Khalil
maher.khalil@fagr.bu.edu.eg
1
Faculty of Agriculture at Moshtohor, Benha University
Gallinacinsare functional analogues of the mammalian beta-defensins and play an important role in the innate immunity against microbial infections in chickens. Salmonella Typhimurium is a gram-negative bacterium that adversely affects both human and animal health. A crossbreeding experiment in chickens was conducted between an Egyptian breed (Fayoumi; F) and an exotic breed (Rhode Island Red; R) to improve the immune genetic response against salmonella in F1 chicks of ½F½R and their reciprocal cross of ½R½F. This study aimed to assess the association between four Gallinacins polymorphic genes (Gal2, Gal3, Gal4 and Gal5) and cecal salmonella bacterial count or antibodies titer (IgA, IgG and IgM). The cecal bacterial count and the antibodies titer were determined in both F1 chicks after pathogenic with S. Typhimurium. The polymorphic genes of Gal2, Gal3, Gal4 and Gal5 were assessed by the restriction fragment length polymorphism technique (PCR-RFLP) using HpyCH4IV, AvaI, AluI and HinfI restriction enzymes, respectively. The Gal2 gene was homozygous for the four genetic groups (FCF, RCR, FCR and RCF), so it was excluded from the association study. Among the four genes studied, the GAL3 gene was found to be significantly (p<0.05) associated with the salmonella cecal bacterial count and the antibodies produced. This gene encourages us to use it in any marker-assisted selection in order to improve and enhance the genetic immune response against salmonella infection in chickens.
https://assjm.journals.ekb.eg/article_57257_3045ffe3beb31e13a3b9e88253de9b5c.pdf
Gallinacin genes
Salmonella
Immune Response
crossbreeding
PCR-RFLP
Poultry
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
35
36
10.21608/assjm.2018.57260
57260
Original Article
Salmonella lipopolysaccharide-induced thymocyte death and thymic atrophy were related to TLR4-FOS/JUN pathway in chicks (Abstract)
Huazhen Liu
lhz219@mail.hzau.edu.cn
1
College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Thymus is the crucial site for T cell development and once believed to be immune privileged. Recently, thymus has gained special attention as it is commonly targeted by infectious agents which may cause pathogenic tolerance and subsequent immunosuppression.We analyzed thymic responses to the challenge with Salmonella typhimurium (STm) or lipopolysaccharide (LPS) derived from STm in chicks. Newly hatched chicks were injected intraperitoneally with 5 × 104 CFU/mL STm or 50 mg/kg LPS. After LPS treatment, maximum thymocyte death (3 ~ 5-fold change) compared to controls was found at 12 h, and maximum loss of thymic weight (35 %) and reduced thymic index (20 %) were found at 36 h. After STm infection, maximum thymocyte death and thymic atrophy occurred at 36 and 72 h, respectively. No significant changes of thymic structure, chT1+ and CD4+/CD8+ T cell ratio were observed in thymus or spleen tissues after LPS treatment. Furthermore, transcriptome analysis revealed important roles for the TLR4-FOS/JUN signaling pathway in thymic injury. Thus, the major process of thymic atrophy in this study first involved activation of transcriptional factors FOS/JUN upon LPS binding to TLR4 that caused release of inflammatory factors, thereby inducing inflammatory responses and DNA damage and ultimately cell cycle arrest and thymic injury. The results indicated that STm and Salmonella LPS could induce acute chick thymic injury. LPS treatment acted faster than STm, TLR4-FOS/JUN pathway may play an important role in LPS induced chick thymic injury.
https://assjm.journals.ekb.eg/article_57260_437b3c2f1559fc06f38239bbd7c98bbf.pdf
Salmonella typhimurium
Thymus injury
chT1
Transcriptome
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
37
46
10.21608/assjm.2018.57306
57306
Original Article
Effect of Dietry Sage Plant Leaves Powder and Ascorbic Acid Supplementation on the Performance of Broiler Chickens under Iraq Conditions
Osama El-Garhy
osama.alsayed@fagr.bu.edu.eg
1
Animal Production Department, Faculty of Agriculture, Benha University, Egypt
A total number of 420 one day-old broiler chicks (Ross 308) were used in this study to evaluate the effect of dietary supplementation of sage plant (Salvia officinalis) leaves powder (SP) and ascorbic acid (Vit. C) On growth performance, and metabolic response of broiler chickens. Chicks were randomly divided into seven experimental groups each of 60 chicks. Chicks of the 1st group fed on basal diets (starter and grower) and considered as a control group, chicks of the 2nd, 3rd and 4th groups fed on basal diets supplemented with SP at a levels of 4, 8 and 12 g/kg diet, respectively. While, chicks of the 5th, 6th and 7th group fed on basal diets supplemented with Vit. C at a level of 200, 300 and 400 mg/kg diet, respectively. The obtained results showed that chicks fed diet supplemented with SP at a level of 12 g/kg diet showed higher improvement in body weight, body weight gain , rate of growth, feed consumption and conversion, blood parameters, economical efficiency, carcass yield and decreased mortality rate followed by those fed diet supplemented with Vit. C at a level of 300 mg/kg diet then by those fed diet supplemented with SP at level of 8 g/kg diet. It could be concluded that SP supplementation at a level of 12 and 8 g/kg diet, respectively and vitamin C at a level of 300 mg/kg diet seemed to be adequate to achieve the favorite results and is being recommended from the economical point of view.
https://assjm.journals.ekb.eg/article_57306_90b56cb5a405e8164b422a94757d9b46.pdf
Broilers – Sage Plant – Ascorbic Acid – Growth Performance
Blood Parameters- Economical Efficiency
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
47
48
10.21608/assjm.2018.57308
57308
Original Article
Application of DNA-Test to expedite breeding of chicken autosexing crosslines (Abstract)
Yanzhang Gong
poultry@mail.hzau.edu.cn
1
College of animal science and technology, Huazhong Agricultural University, Wuhan, China
It is necessary in modern chicken meat and egg production that the sex of chicks is distinguished at hatching, because only females are raised in egg-type commercial crosslines and it is encouraged to feed separately the males and females in meat-type lines. Traditionally, vent sexing, in which sex of hatching chicks is identified by specially trained, experienced sexers through visually observing if there exists rudimentary sex organ in the cloaca, is used. Currently, phenotypic markers controlled by sex-linked gene are used for autosexing in some commercial crosslines, and the characteristics used most popularly are early vs. late-feathering, silver vs. golden color of down, etc. However, there is limited time period(just during the infancy because the down is observed) to accurately identified the phenotype(and to deduce the genotype), and in the same time there would be some identification error. These result in longer time(at least 2-3 generations) for accomplishing the combination of lines, low accuracy for autosexing and high cost. We developed approaches to identify the phenotype in early/late feathering locus and genotype in silver/golden locus based on their controlling genes by DNA-test, and these broke through the time limit to distinguish,and increased the identification accuracy, and as a result, the breeding time of autosexing crosslines and the cost were reduced. A double PCR was used to distinguish the phenotype of early/late feathering based on the 176kb copy number variation(CNV) and this approach was to utilize in breeding the Xinhua layer no.2(which was approved by national committee of animal genetic resources in 2016), the Baoqiang layer and Sanyi green shell layer crosslines. Based on the SLC45A2 which controlling the phenotype of Silver/golden, a PCR approach was developed to identify the genotype in this locus and utilized in the breeding of Jingyang(a dual types) crosslines. By using these approaches, the time needed for accomplishing the combination of lines could be saved at least 1/3(and for cost, at least 1/4), the accuracy of autosexing could arrive to above 99%. In addition, to decrease the time for DNA-test, the way of isolation of DNA, which would be used as DNA model in PCR, was improved and this made the time decreased to 3 hours from at least 10 hours.
https://assjm.journals.ekb.eg/article_57308_fb0ea91c0b603945d6cf5757c64854fe.pdf
chicken autosexing DNA-Test
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
49
56
10.21608/assjm.2018.57311
57311
Original Article
Effects of dietary malic acid on growth, nutrient utilization and serum biochemical indices of Nile tilapia (Oreochromis niloticus) fingerlings
magdy soltan
magdy.soltan@fagr.bu.edu.eg
1
Fac. Agric. Moshtohor, Benha University, Egypt1
A 90 days feeding trial was conducted to investigate the effect of dietary supplementation with malic acid on growth, nutrient utilization and some hematological parameters of Nile tilapia (Oreochromis niloticus). Three isonitrogenous (300 g CP kg−1) and isocaloric (14.90 MJ gross energy kg−1) diets were formulated. The control diet (D1) contained no added of malic acid. Two diets were supplemented with 0.5% (D2) and 1.0% (D3) malic acid. A set of 180 fish of O. niloticus L. mono-sex male fingerlings average initial weight of 7.56 ±0.06 g were randomly distributed into nine plastic tanks (0.45 m3 for each) representing the three treatments studied. Twenty fish were randomly stocked into each tank with three replications for each treatment. At the end of the feeding trial, O. niloticus offered the control diet showed lower growth and feed utilization than all those fed malic acid supplemented diets. Fish fed the diet D3 showed the highest body weight (BW) weight gain (WG), specific growth rate (SGR), feed intake (FI), and the best feed conversion ratio (FCR) and protein efficiency ratio (PER). Supplementation of malic acid (5 g or 10 g kg-1) increased hemoglobin (Hb), hematocrit (Htc) and red blood cell count (RBCs) of Nile tilapia, but white blood cells (WBCs) of did not significantly (P>0.05) affected by malic acid supplementation. The highest level of Hb, Htc and RBC was recorded by fish fed 10 g kg-1. In the same direction supplementation the basal diet with 5 g or 10 g kg-1 malic acid significantly increased the levels of serum Albumin u/L (P<0.001), Globulin (P<0.05) and total protein (P<0.001). The present observations suggest that, growth and feed utilization of O. niloticus can be enhanced with a supplementation of 1.0% malic acid in the diet.
https://assjm.journals.ekb.eg/article_57311_a883fdcae1895a42e078dfeb58f5f850.pdf
Growth
nutrient
organic acid
Nile tilapia
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
57
62
10.21608/assjm.2018.57314
57314
Original Article
Effect of seasons on productive performance, metabolic changes and immunity response in rabbits
Yasmein A.
jasmein2004@hotmail.com
1
Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
The present study was carried out to investigate the effect of season on growth performance traits, metabolite assays and gene expression for innate immune in response to vaccination against pasteurellosis. APRI line, weaned females rabbits of were used in this study a local population. Rabbits weaned at 35 days and introduced in the experiment. Weight at 5 weeks considered as the beginning and all rabbits were weighed every 7 days until 12 weeks old. The feed consumption measured at the same weekly interval. Immunity response was studied by vaccination with Pasteurella multocida at 8 and 10 weeks of age as challenge experiment and the blood samples taken at 8 , 10 and 12 weeks of age. Gene expressions for interleukin-6 (IL-6) and toll-like receptor-4 (TLR-4) were assayed by Real Time-PCR. Metabolite assays included glucose, triglycerides, and urea nitrogen and blood samples were taken at 8, 10 and 12 weeks of age, respectively. The body weights in winter at W5, W8and W12 of age were higher than those in other seasons. Season had significant (P<0.05) influence on daily gain and feed intake. Triglycerides were significantly higher (P< 0·05) in rabbits during summer. From summer to spring season, the urea values gradually increased significant in rabbits. Vaccination with Pasteurella multocida led to an increase in expression for IL-6 in autumn and winter. Expression for IL-6 in spring was lowest the values. Gene expression of TLR4 in rabbits under different seasons had not significantly different. With vaccination by Pasteurella multocida, it is recommended by using additives to enhance immunity during summer and spring in rabbit.
https://assjm.journals.ekb.eg/article_57314_1431014b675b94e82bb8b856e1ecb9c4.pdf
Rabbit
season
Gene expression
immunity
metabolic changes
Growth
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
63
64
10.21608/assjm.2018.57319
57319
Original Article
Genetic Diversity and Population Structure of Native Geese in Egypt Using Microsatellite Markers (Abstract)
E. Abdel-Kafy
sayedabdkaffy@yahoo.com
1
Animal Production Research Institute, Agricultural Research Centre, Dokki - Giza - Egypt
The native geese are valuable sources of genetic material because of their adaptation to harsh conditions and their tolerance to a range of diseases. Assessment of genetic diversity is important to identify the native geese populations that are useful for food security and rural development. Geese can utilize cheap raw materials and produce significant amount of palatable meat. Additionally, geese had ahistoric importance in Egypt as they found on the walls of ancient temples. In the present study, the genetic diversity and relationships of three Egyptian geese populations were assessed using 12 labeled microsatellite markers (CK13, Aalц1, ANS02, ANS02, ZAAS004, ZAAS018, ZAAS023, ZAAS025, ZAAS036, ZAAS052, ZAAS152, and ZAAS173). Genomic DNA was isolated from blood samples of 90 geese collected from three Egyptian governorates: Kafer El-Sheikh, El-Fayoum and Luxor. The PCR products were electrophoresed on an ABI 3500xl DNA Sequencer. The basic measures of genetic diversity and phylogenetic trees were assessed using bioinformatics softwares. The information from this study should be useful for genetic characterization and for developing conservation programs of this agriculturally and commercial important species. The results confirmed also that the used microsatellite markers in assessing the genetic diversity among Egyptian geese are applicable.
https://assjm.journals.ekb.eg/article_57319_97533b8a4f771d5953c2ecffe6eaa3d4.pdf
Indigenous Egyptian geese
Genetic diversity
Microsatellite
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
65
66
10.21608/assjm.2018.57320
57320
Original Article
Comparative transcriptome analysis of zebrafish (Danio rerio) brain and spleen infected with Spring Viremia of Carp Virus (Abstract)
Xueqin liu
xueqinliu@mail.hzau.edu.cn
1
College of Fisheries, Huazhong Agricultural University, Wuhan, China
SVCV is the pathogen of spring viremia of carp (SVC) and often causes acute hemorrhagic symptoms in various kinds of cyprinids and induces serious environmental and economic losses. However, the molecular mechanisms of infection remain poorly understood, especially at the individual level. In this study, zebrafish was employed as the infection model to explore the pathogenesis of SVCV. 4 groups of zebrafish tissues were set and RNA sequencing (RNA-Seq) technology was employed to analyze the differentially expressed genes (DEGs) after SVCV-infection. A total of 360,971,498 clean reads were obtained from 12 samples, 382 DEGs in the brain and 926 DEGs in the spleen were identified. These DEGs were annotated into three ontologies after gene ontology (GO) enrichment analysis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these DEGs were primarily related to Influenza A pathway and Herpes simplex infection pathway in brain and Tuberculosis and Toxoplasmosis pathways in spleen, and all of these pathways may be involved in response to pathogen invasion. At the same time, 3’ and 5’ alternative splicing (AS) events were significantly up-regulated in the spleen. The transcriptome analysis results demonstrated changes and tissue-specific influences caused by SVCV in vivo, which provided us with more information to understand the complex relationships between SVCV and its host.
https://assjm.journals.ekb.eg/article_57320_0aeebe5d2d0760eac7f76013a4cdbd7d.pdf
Molecular mechanisms
Infection
zebrafish
SAVCV-infection
Herpes infection
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
67
68
10.21608/assjm.2018.57322
57322
Original Article
Molecular analysis of milk yield and components associated with some genetic markers in dairy cattle.
Maher Khalil
maher.khalil@fagr.bu.edu.eg
1
Faculty of Agriculture at Moshtohor, Benha University
Prolactin and Lactoferrine genes related with milk yield and milk components were analyzed for 180 milking cows. Genotyping for these two genes were investigated to detect their associations with yields of milk, fat and protein and age at first calving (AFC). About 38 local cows from Serw farm (Baladi) in Damietta governorate, 62 Friesian cows from Sakha farm and 80 from El Karada farm in Kafr El-Sheikh. Blood samples were collected from all cows in EDTA tubes and salting out method was used to extract DNA from these blood samples. PCR RFLP method was used to get single nucleotide polymorphism (SNP) by PCR technique. Three enzymes were used to restrict DNA product (HINF1 for lactoferrin gene, MRON1 and SML1 restriction enzymes for prolactin gene).
There were significant differences among three genotypes (AA, AB and BB) detected by restriction enzyme HINF1 in Frisian cattle for milk, fat, protein yield traits but not significance for AFC trait. The genotype AB was the best one for milk, fat and protein yields. In local cattle at Serw farm, there was no significance differences among three genotypes for milk, fat and protein yield, but for age at first calving there were significance differences. The genotypes from restriction enzyme MRON1 were two genotypes (CD and DD) for El-Qarada herd. There were significant differences between the two genotypes in Frisian herd for milk, fat, protein yields, but significance for AFC trait. In Serw herd, there were not significant differences among the three genotypes for milk, fat and protein yields, but AFC was significance. Genotypes from restriction enzyme SML1 were GG, GT and TT. There were significance differences among three genotypes in Friesian herd for milk, fat and protein yields, while in AFC there were non-significance differences. In Sero herd, differences in genotyping among four traits were not significant.
https://assjm.journals.ekb.eg/article_57322_7d6499a8f1f0a0223f5ccdf85baffcc4.pdf
dairy cattle
Molecular analysis
PCR RFLP
Milk yield and components
Prolactin and Lactoferrine genes
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
69
74
10.21608/assjm.2018.57328
57328
Original Article
Effect of melatonin supplementation to the maturation media on in vitro cumulus cells expansion and nuclear maturation rates of buffalo oocytes
Asmaa Fathy
asmaa.nebar@yahoo.com
1
2Animal Production Department, faculty of Agriculture, Menoufia University, Egypt
The aim of this study is to determine the effect of the melatonin addition to the maturation medium (MM) on in vitro maturation of buffalo oocytes (IVM). Maturation is evaluated by the cumulus cells expansion and nuclear maturation. The cumulus oocyte complexs (COCs) were harvested by aspiration of antral follicles (2-8 mm diameter) of slaughter buffalo ovaries, COCs with homogeneous cytoplasm and at least three layers of cumulus cells were selected. The COCs were incubated for 24h in TCM–199 medium with melatonin (10, 30 or 50ng/ml) or without addition (control). After 24 h in culture media, the maturation rate of oocytes was determined by evaluating the expansion degree of the cumulus-oocyte complex (COCs) in each treatment, then classified into: fully expanded, partially expanded or not expanded, and byevaluation stages of oocyte nuclear maturation (GV, GVBD, MI, MII or degenerated). Results illustrate that supplementation maturation media with 10 and 30, 50 ng/ml melatonin resulted in increasing the cumulus cell expansion of buffaloes oocytes by 86.4, 82.3, 83.2 %, respectively, as compared with oocytes cultured with melatonin - free medium (73.3%), The respective differences were significant. Majority of this improvement in the expansion rates of oocytes was observed in the rate of fully expanded oocytes, On the contrary, the rate of immature (unexpanded) oocytes incubated in melatonin-free medium was significantly (P<0.01) higher (26.7%) than those incubated in maturation media with 10 or 30 or 50 ng/ml melatonin (13.8, 17.7, 16.9 %, respectively). Moreover, results indicated that addition of melatonin to the maturation medium significantly improved the percentage of oocytes of first polar body (matured to MII) as compared with those incubated without melatonin, the highest percentage of those oocytes was obtained with 10 ng/ml melatonin (58.7 %), however, the least percentage was in those oocytes cultured with melatonin - free medium (48.6%) with significant differences (P<0.05). On the other side, the percentage of oocytes of GVBD, MI or degenerated stages did not significantly affected with melatonin addition (10. 30 or 50 ng/ml) to culture media as compared with those cultured without melatonin medium. It could be concluded that addition of melatonin to the MM improved cumulus expansion and nuclear maturation rate of buffalo oocytes.
https://assjm.journals.ekb.eg/article_57328_21cc79f05917d24a050b201ae5a6768d.pdf
Buffalo
IVM
oocyte maturation
Melatonin
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
75
76
10.21608/assjm.2018.57331
57331
Original Article
Development and validation of a sensitive monoclonal antibody-based ic-ELISA for the aflatoxin M1 in milk (Abstract)
Dapeng Peng
pengdapeng@mail.hzau.edu.cn
1
College of veterinary medicine, Huazhong Agricultural University, Wuhan, China
Aflatoxin M1 (AFM1), a hydroxylated metabolite of aflatoxin B1 (AFB1), possesses the carcinogenic, mutagenic, and toxic activities. To protect the health of animals and humans, it is clear that the need for an effective residue-monitoring programme to detect the AFM1 residues. In this study, a modified procedure was used to prepare the hapten AFB1 and AFM1 derivatives. Then, the prepared antigen AFM1-CMO-KLH was used to inoculate female Balb/c mice to prepare a sensitive monoclonal antibody (mAb) against AFM1. After cell fusion and culture several times, the hybridoma cell line, 3D8, which was of the IgG1 isotype, was selected to obtain a highly sensitive mAb. The obtained 3D8 mAb displayed an IC50 value of 64.75 ng L-1 for AFM1 and did not exhibit measurable cross-reactivity with other aflatoxins and antibiotics. Based on this mAb, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established that utilizes simple sample preparation and clean-up methods. The decision limit (CCα, α = 1%), detection capability (CCβ, β = 5%), and LOQ value for the AFM1 matrix calibration method were 24 ng L-1, 27.5 ng L-1, and 35 ng L-1 in the milk matrices, respectively. The AFM1 recovery ranged from 85.3% to 107.6%. The CVs were less than 13.8%. A positive correlation (r > 0.99) was observed between the ic-ELISA and HPLC-MS/MS results. This ic-ELISA would be a useful tool for screening the AFM1 residues in milk.
https://assjm.journals.ekb.eg/article_57331_cdb012167338406364a61840743583c8.pdf
Monoclonal antibody (mAb)
Aflatoxin M1
Milk
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
77
78
10.21608/assjm.2018.57332
57332
Original Article
Development of a Novel Genetically Modified Bioluminescent Bacteria-Based Assay for Detection of Fluoroquinolones in Animal Derived Foods (Abstract)
Guyue Cheng
chengguyue@mail.hzau.edu.cn
1
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Fluoroquinolones (FQNs) are broad-spectrum antibacterial agents widely used in animal husbandry and aquaculture. The residues and antimicrobial resistance of such antibiotics are the major public health concern. In order to realize multi-analyte detection of FQN residues, a genetically modified bacterium, E. coli pK12 harboring plasmid pRecAlux3, was constructed in this study to develop a bioluminescent bacteria-based assay (BLBA) for the detection of FQNs in animal derived foods. This assay was based on the principle of induction of SOS response by FQNs via inducing the recA promoter-fused luciferase reporter gene existing on the plasmid pRecAlux3. E. coli pK12 was able to recognize 11 FQNs, including difloxacin, enrofloxacin, ciprofloxacin, sarafloxacin, norfloxacin, danofloxacin, ofloxacin, pefloxacin, lomefloxacin, marbofloxacin and orbifloxacin. This method could be applied to 11 edible tissues, including milk, fish muscle, the muscles, livers and kidneys of cattle, chickens and pigs, with very simple and rapid sample extraction procedure using only phosphate-buffered saline. The limits of detection of the FQNs were between 12.5 and 100 μg kg-1, all of which were lower than the maximum residue limits. Most of the recoveries of FQNs were in the range of 60 to120% and the inter-assay coefficients of variation were less than 30%. This method confirmed by HPLC is reliable and can be used as both screening test and semiquantitative assay, when the identity of a single type of FQN is known.
https://assjm.journals.ekb.eg/article_57332_a8c25ecd16466911a299994bbf9ec40f.pdf
Fluoroquinolones (FQNs)
Animal Derived Foods
bioluminescent bacteria
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
79
80
10.21608/assjm.2018.57333
57333
Original Article
Effects of Tetramethylpyrazine and Icariin on tibial dyschondroplasia incidence, tibial angiogenesis, performance and characteristics via regulating HIF-1α/VEGF/WNT4 signaling in chickens (Abstract)
Jiakui Li
lijk210@sina.com
1
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China College of Animals Husbandry and Veterinary Medicine, Tibet Agricultural and Animal
Tibal dyschondroplasia (TD) is main bone problem in fast growing poultry birds that effect proximal growth plate of tibia bone. TD is broadly defined as non-vascularized, non-mineralized, and enlarged GP with tibia bone deformation and lameness. Tetramethylpyrazine (TMP) is widely used to treat neurovascular disorders and pulmonary hypertension, whereas icariin (Epimedium sagittatum) is a traditional Chinese medicine, which is commonly practiced in the treatment of various bone diseases. But, there are no reports about the promoting effects of TMP and icariin against TD chickens. Therefore, a total of 280 broiler chicks were equally divided into four groups; Control, TD, TMP and icariin groups. All groups were offered ad libitum regular diet. The TD, TMP and icariin group were fed same diet as control group but with the addition of tetramethyl thiuram disulphide (thiram) 50 mg/kg of feed from day 3-7 post hatch to induce TD. After induction of TD on day 8 the TMP and icariin group was fed with standard normal diet with the addition of 30 mg/kg/day TMP and 10 mg/kg/day icariin respectively, until the end of experiment. Whereas, TD group was given standard normal diet without adding thiram, just like control group. During the experiment mortality rate, chicken performance indicators (daily weight, average daily feed intake, average daily weight gain and feed conversion ratio), tibia bone indicators (weight, length, width of tibial and the size of GP) in addition to gene and protein expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and wingless type member 4 (WNT4) were examined. The results showed that TMP and icariin administration restore the GP width, increase growth performance, and mitigated the lameness in broiler chickens. The expression of HIF-1α, VEGF increased, while WNT4 decreased significantly in TD affected thiram induced chicks. The TMP treatment significantly down-regulated HIF-1α and VEGF genes and proteins expressions, whereas icariin treatment up-regulated WNT4 and down regulated VEGF gene and protein expressions significantly. This is the first study that explains the effect of TMP and icariin in thiram-induced TD chickens. The present study demonstrates that TMP and icariin play important role in angiogenesis during the impairment and recovery of GP in TD by regulation of the HIF-1α, VEGF and WNT4 signaling in chickens.
https://assjm.journals.ekb.eg/article_57333_dfdad3489bcf042b0090e2e702469045.pdf
Tibial dyschondroplasia
growth plate
chickens
TMP
icariin
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
81
88
10.21608/assjm.2018.57335
57335
Original Article
Effect of different protein and energy levels on growth performance and body composition of red tilapia
Mohamed Abou Zead
myabouzead@yahoo.com
1
World fish, Regional Research Center for Africa and west Asia, Abbassa, Sharkia, Egypt.
A 8-weeks feeding trial was conducted to explain the most suitable levels of protein and energy for red tilapia fry reared in fresh water. Nine experimental diets of 25, 30 and 35% crude protein and within each level, three levels of metabolizable energy (ME) 240, 280 and 320 kcal/100 g diets were formulated. Red tilapia fry (0.77 g) fed the experimental diets at a daily rate of 20% (during the 1st month) and then decreased to 10% (2nd month) of the total biomass. The obtained results showed that, increasing dietary protein and energy levels significantly improved all growth parameters (body weight, body length, weight gain and specific growth rate) of red tilapia fry. Fish group fed the diet contained 35% crude protein and 320 Kcal ME/100 g showed the highest final body weight, body length, weight gain and specific growth rate while fish fed the diet contained 25% crude protein and 240 kcal ME/100 g showed the lowest growth parameters. It is also found that increasing protein or energy levels in red tilapia fry diets significantly increased feed intake (FI) and significantly improved feed conversion ratio (FCR) and protein efficiency ratio (PER). The best feed efficiency parameters (feed conversion ratio and protein efficiency ratio) were obtained for fish group fed the diet containing 35% crude protein and 320 kcal ME/100 g while the diet contained 25% crude protein and 320 kcal ME/100 g diet had the worst FCR. Increasing dietary protein and energy levels in red tilapia fry diets significantly decreased protein and increased fat contents of red tilapia bodies. Generally, based on the present results, the experimental diet containing 35% crude protein and 320 kcal ME/100 g considered the most suitable diet for red tilapia fry reared in fresh water.
https://assjm.journals.ekb.eg/article_57335_c38b2e8ba4e6634b4232f4269562e8e8.pdf
Red Tilapia
protein
energy
Growth
nutrient utilization
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
89
90
10.21608/assjm.2018.57337
57337
Original Article
Function and mechanism of map4k4 genes on milk traits and scs between dairy cow and buffalo (Abstract)
Shujun Zhang
sjxiaozhang@mail.hzau.edu.cn
1
Huazhong Agricultural University, Wuhan, China
1. The variation of MAP4K4 gene has a significant effect on the number of somatic cells in milk. The objective of this study is to explore the association of single nucleotide polymorphisms present in MAP4K4 gene with different milk traits in dairy cows. Milk production traits including milk yield, fat percentage, and protein percentage from 500 Holstein cows were collected by using 305 days lactation records. Association between MAP4K4 genotype and different traits and Somatic Cell Score (SCS) was performed using General Linear Regression Model of R. Two SNPs at exon 18 (c.2061T>G and c.2196T>C) with genotype TT in both SNPs were found significantly higher for somatic SCS. Thus, MAP4K4 gene could be a useful candidate gene for mastitis and the identified polymorphisms might potentially be strong genetic markers.
2. The promoter sequence of MAP4K4 gene in dairy cattle and buffalo significantly affected the expression level of MAP4K4. we identified the differences between the core promoter region of the cow and buffalo, the promoter region from -1100 to -778 bp was essential for cow-MAP4K4 while -608 to +176 bp was essential for the buffalo-MAP4K4 that the two regions contained the core functional promoter to maintain the promoter activity. The core promoter activity of cow-MAP4K4 was significantly higher (P<0.05) than buffalo-MAP4K4, mightimply the susceptibility of mastitis and other inflammatory disease more in cow than buffalo.
https://assjm.journals.ekb.eg/article_57337_7b8ea9b51dc9ff2d9dc7c0dfa6f5ad0a.pdf
mastitis
somatic cell score (SCS)
MAP4K4
SNP
Core Promoter
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
91
98
10.21608/assjm.2018.57338
57338
Original Article
Effect of housing system and dietary biotin supplementation on 2- Egg quality traits and some blood constituents
Hamada Hamada
hamada.okasha@fagr.bu.edu.eg
1
Animal prod. dept., Faculty of Agriculture, Benha University
The present study was aimed to investigate the effect of housing system and dietary biotin supplementation on productive and metabolic performance of Benha line chickens. A total number of 224 chickens 20 weeks old and mean of body weight (1742 ± 20.1), were equally divided into two groups in a factorial arrangement design (2x4x3). Pullets of the first group were housed in cages of laying battery (with artificial insemination) two birds were kept per cage of 48×40cm (960cm2/bird). Pullets of the second group were kept on deep litter laying houses (with natural mating) in a density of 900cm2/ bird. Chickens of each group were then subdivided into four sub groups each of 25 females and 3 males according to dietary biotin supplementation. Pullets of the 1st sub group were fed on basal layer diet and considered as control group, the 2nd, 3rd and 4th sub groups were fed on basal diet supplemented with biotin at a level of 100, 150 and 200µg/kg diet, respectively. The results obtained showed that, pullets housed in cages improved significantly relative weights of egg shell and albumin, indexes of egg shape, albumin and yolk, hatchability percentage and chick weight athatch, it also decreased early, mid and late embryonic mortality, plasma total proteins, aspartate aminotransferase, creatinine and plasma uric acid. However pullets housed on deep litter improved yolk index, Haugh unit, fertility percentage, plasma levels of albumin, globulin and alanine aminotransferase. Biotin supplementation to layer diets at a level of 150 µg / kg diet increased relative weight of egg shell and albumin, yolk index, fertility and hatchability percentage and decreased early, mid and late embryonic mortality. Chick weightat hatch, egg shape index, relative weight of albumin and plasma level of albumin significantly increased by adding 100 µg biotin to layers diets. It could be concluded that biotin supplementation at a level of 150 and 100 µg/ kg in layer diet and reared in battery cages and on deep litter system, respectively seemed to be adequate to achieve the favorable results.
https://assjm.journals.ekb.eg/article_57338_bc380b2032c3e64f7425af80fdbb6581.pdf
laying hens
housing system
biotin
egg quality
Blood
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
99
100
10.21608/assjm.2018.61897
61897
Original Article
Bio-technology of using photogenic feed additives in poultry diets (Abstract)
shaker Abd EL-Latif
profshaker71@yahoo.com
1
Department of Animal Production, Faculty of Agriculture, Minia University, Minia, Egypt
The use of feed additives is usually subject to restrictive regulations. In general, they are considered as products applied by the farmer to healthy animals for a nutritional purpose on a permanent basis (i.e., during the entire production period of the respective species and category), in contrast to veterinary drugs (applied for prophylaxis and therapy of diagnosed health problems under veterinarian control for a limited time period, partially associated with a waiting period). Plant species from the families of Zingiberaceae (e.g., ginger and curcuma) and Umbelliferae (e.g., anise and coriander), as well as plants rich in flavonoids (e.g., green tea) and anthocyans (e.g., many fruits), are also described as exerting antioxidative properties. Furthermore, pepper (Piper nigrum), red pepper (Capsicum annuum L.), and chili (Capsicum frutescene) contain antioxidative components. In many of these plants, parts of the active substances are highly odorous or may taste hot or pungent, which may restrict their use for animal feeding purposes. The antioxidant property of many phytogenic compounds may be assumed to contribute to protection of feed lipids from oxidative damage, such as the antioxidants usually added to diets (e.g., α-tocopheryl acetate or butylated hydroxytoluene). Although this aspect has not been explicitly investigated poultry feeds, there is a wide practice of successfully using essential oils, especially those from the Labiatae plant family, as natural antioxidants in human food, as well as in the feed of companion animals. The objective of this review was to evaluate the Bio-technology of using photogenic feed additives in poultry diets.
https://assjm.journals.ekb.eg/article_61897_9652ef23d2fe8b89df571ef414904607.pdf
Bio-technology
Feed additives
Poultry
Diets
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
101
102
10.21608/assjm.2018.64888
64888
Original Article
Growth performance and carcass quality of broilers as affected by light flashes program in relation to dietary vitamin d3 levels (Abstract)
M. Farghly
farghly20002000@aun.edu.eg
1
Dept. of poultry Production, Fac. Of Agric., Univ. of Assiut, Egypt
This study was undertaken to determine the effect of dietary vitamin D supplementation in relation to light flash program on the growth performance, carcass traits, meat quality, some blood parameters and economic efficiency of broiler chickens. Therefore, 270 birds were randomly distributed into three main groups and housed in floor pens and reared under continues light for 24 hrs per day; 16 hrs continues light +8 h flash light/day and 8 hrs continues light +16 hrs flash light/day, respectively. Each main group were divided into 3 equal groups (60 birds each) and received 0, 100 and 200 ppm vitamin D from 4 wks till six weeks of age. Main results could be summarized as follows: light flash program significantly affected body weight, daily weight gain, feed conversion, dressed carcass, some blood traits as T3 hormone, leg problems, lymphoid organs and body temperature. However, it had insignificant effects on most meat quality traits. In addition, the body weight and body weight gain were significantly increased at all levels of dietary vitamin D. The liver, bursa and breast weights (expressed as percent of body weight) increased significantly with diet containing 100 or 200 ppm vitamin D. Meat quality traits, bone measurements and plumage conditions significantly affected by all levels of dietary vitamin D. Plasma protein, glucose, AST and ALT activities increased significantly in birds fed diets added 200 ppm vitamin D levels. Economic efficiency of the broiler chickens was precisely improved at levels of 100 ppm dietary vitamin D with second program of flash light (16 hrs continues light +8 h flash light/day) .
https://assjm.journals.ekb.eg/article_64888_d4e052fc5280d95bbfa6be1488ec3a0c.pdf
light flash and vitamin D
performance
broiler chickens)
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
103
104
10.21608/assjm.2018.65117
65117
Original Article
The Agrobacterium protein VirD5 is required for efficient Agrobacterium infection (Abstract)
Shaojuan Zhang
mzluo@mail.hzau.edu.cn
1
National Key Laboratory of Crop Genetic Improvement and College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
Agrobacterium exports at least five virulence proteins (VirE2, VirE3, VirF, VirD2, VirD5) into host cells and hijacks some host plant factors to facilitate its transformation process. We found that Agrobacterium VirD5 exhibits transcriptional activation activity in yeast, is located in the plant cell nucleus, and forms homodimers. A specific VirD5-bound DNA element designated D5RE (VirD5 response element) was identified. We also found that VirD5 interacted directly with Arabidopsis AtVIP1 and AtVIP2 (VirE2 Interacting Protein 1 & 2); competes with VBF for binding to AtVIP1 and stabilizes AtVIP1 and VirE2 in the cell-free degradation system; affects cap-binding proteins (CBP20 and CBP80) for binding to VIP2. Deleting virD5 from Agrobacterium reduced its tumor formation ability and stable transformation efficiency but did not affect the transient transformation efficiency. The tumorigenesis efficiency for cbp80 mutant was not significantly changed, but that for cbp20, cbp20cbp80 mutants were significantly increased. This work experimentally demonstrated that VirD5 is required for efficient Agrobacterium infection.
https://assjm.journals.ekb.eg/article_65117_1ba4865c8ea303551de8f65b3dcd4fb8.pdf
Agrobacterium
virulence proteins
cap-binding proteins
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
105
114
10.21608/assjm.2018.65118
65118
Original Article
Response of some rice varieties to different doses of X rays on the yield and its related characters
M. Abo- Youssef
1
Rice Research Department, Field Crops Research Institute, Agriculture Research Center, Egypt.
This experiment was carried out at the experimental farm of Sakha Agricultural Research Station, Kafr Elsheikh , Egypt during 2016 and 2017 seasons, the materials including six rice varieties treated with three doses of X rays. The experimental design was a split plot design, the main plots were devoted to doses of X-rays, while, sub plots were devoted to rice varieties with three replications, the date of sowing for M1 was after seed treated during May 1st 2016 and M2 was planted in 1 st May during 2017 season, the data were recorded on total duration, plant height and yield components. The results showed that all the studied characters affected by different doses of X rays and the dose (300 Kr) recorded the desirable values for most studied characters. Moreover, the variety Giza 171 was highly affected by the different doses of X rays, whereas, the high dose of X rays recorded the shortest plant height compared to Giza 159. Also, GZ6296 was highly affected by different doses of X rays especially for seed set % which referred to the genetic makeup (Indica / japonica) for this line indicating the occurrence of some rearrangement for the genetic background. On the other side, M2 generation recorded highest values for all the studied characters of all the treated varieties compared to M1 generation, meaning that, during M2 generation some different rearrangement was occurred on the molecular levels for the genetic structure of the rice varieties thus exchanging the gene expression for the morphological and yield characters. In M1 generation over the all genotypes, irradiation doses significantly decrease most of the studied characters; in contrast, total duration and plant height were significantly increased by irradiation treatments in M1 generation. In M2 generation the irradiation doses significantly increased the mean value over all the genotypes for number of panicles/ plant, panicle length, panicle weight, seed set %, 1000 grain weight, grain yield / plant and harvest index %, vice versa for total duration and plant height decreased . Genotype x dose treatment interaction was significant for all the traits, these results suggested, that promising lines of the varieties Giza 171 and Giza 159, E. Yasmin could be used to improve some characters such as, short stature, early maturity and highly grain yield.
https://assjm.journals.ekb.eg/article_65118_f875fa0c158ae20523ff2820c703652e.pdf
Rice varieties
Mutation
X-rays
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
115
116
10.21608/assjm.2018.65119
65119
Original Article
The Possibility of Replacing Puddled Transplanted Flooded Rice with Direct-seeded rice in Central China (Abstract)
Lixiao Nie
nielixiao@mail.hzau.edu.cn
1
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
Replacement of puddled transplanted rice (PTR) by direct-seeded rice (DSR) can potentially reduce consumption of resources and decrease emissions of greenhouse gases while maintaining grain yields in central China. However, direct seeding has not been widely adopted in this region. This review was undertaken to better understand the problems and opportunities for replacing PTR with DSR in central China. The seeding rate, crop growth, grain yield, water productivity, nitrogen use efficiency, greenhouse gas emissions and root development was compared between DSR and PTR. With good water management, grain yield of DSR is similar to or higher than yield of PTR while increasing irrigation water productivity and nitrogen use efficiency, reducing greenhouse gas emissions, and decreasing labor requirement. However, problems that include lodging, weak root development, weed infestations and poor crop establishment under drought, waterlogging, or chilling stresses might limit wide-scale adoption of DSR in central China. Varieties bred and selected for direct seeding, guidelines for improved nutrition, water, and weed management practices, and the development of suitable planting machines and sowing management for DSR are needed. In addition, incorporating DSR into the double season rice or ratoon rice systems could be promising strategies to increase rice production in central China.
https://assjm.journals.ekb.eg/article_65119_f68b9c1d0a7a304a1478eaa2d321c732.pdf
Direct-seeded rice
Puddled Transplanted Flooded Rice
central China
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
117
118
10.21608/assjm.2018.65120
65120
Original Article
Current status of rice brown planthopper against insecticides in China (Abstract)
Li Jianhong
jianhl@mail.hzau.edu.cn
1
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
The brown planthopper (Nilaparvata lugens Stål) is one of the most destructive pest of rice crops throughout Asia, and this pest has developed high resistance to many groups of insecticides following their heavy and frequent application. In order to investigate the current status of resistance to frequently used insecticides, a total of fifty-three field populations of N. lugens were collected in nine Chinese provinces, and resistance to insecticides was monitored from 2013 to 2017. The result showed that field populations of N. lugens had developed susceptible to low resistance levels to sulfoxaflor (RR 0.8-8.0), susceptible to moderate resistance levels to etofenprox (RR 1.0-11.2), triflumezopyrim (RR 1.2-17.1) and nitenpyram (RR 1.2-17.3), low to moderate resistance levels to chlorpyrifos (RR 7.7-57.4), low to high resistance levels to clothianidin (RR 6.1-138.8) and dinotefuran (RR 6.4-187.0), and moderate to high resistance levels to isoprocarb (RR 15.4-113.9) and thiamethoxam (RR 25.9-1025.6). Furthermore, all fifty-three field populations of N. lugens had developed high resistance levels to imidacloprid (RR 220.4-6324.0) and buprofezin (RR 306.4-11573.2). Except etofenprox, nitenpyram and chlorpyrifos, the resistance ratio of N. lugens to other insecticides all showed an increasing trend from 2013 to 2017. The obvious increase in resistance to widely applied insecticides indicates that insecticide resistance management strategies are urgently needed to prevent or delay further increase of insecticide resistance in N. lugens.
https://assjm.journals.ekb.eg/article_65120_28ef1d3028f17c50f4a20f20b254d0c5.pdf
Brown planthopper
Insecticides
China
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
119
126
10.21608/assjm.2018.65121
65121
Original Article
Genetic engineering to improve β-carotene content in pepper
Mahran El Nagar
mahran.ashry@fagr.bu.edu.eg
1
Horticulture Department, Faculty of Agriculture Research Park (Biotechnology Lab.), Benha University, Egypt.
A laboratory study was conducted to develop a broadly applicable in vitro regeneration and transformation methods for increasing β-carotene content in pepper fruits. Therefore, two different pepper genotypes were analyzed with regard to their efficiency for regeneration and genetic transformation in vitro. Cotyledons were used in vitro from young seedlings as target tissue for regeneration and transformation of pepper. Over expression of β-Lcy gene via Agrobacterium-mediated transformation was used. The presence of the transgene was assayed on leaf DNA via PCR and biochemical analysis using HPLC. Results strongly showed that there were significant differences between pepper genotypes. The transgenic pepper plants showed a significant increase in fruit β-carotene content reaching 7 to 10 folds according to the genotype. The fruits from these plants displayed different colour phenotypes, from orange to orange-red. The difference in the fruit colour phenotypes reflects the beta-carotene ratio, which ranges from 4.5 mg/100g dw in the wild plants to 45.0 in the transgenic plants of genotype Balady during maturity stage. Concerning to the genotype Topepo rosso, the β-carotene content was increased from 5.2 mg/100g dw in the wild plants to 36.39 mg/100g dw in the transgenic plants during mature stage.
https://assjm.journals.ekb.eg/article_65121_a657c7f4e80ceff6a85861beb496ddc6.pdf
pepper
in vitro regeneration
genetic transformation
β-Lcy
Carotenoids
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
127
136
10.21608/assjm.2018.65122
65122
Original Article
Micro propagation of Date Palm (Phoenix dactylifera L.) var. Zaghlol via Direct Organogenesis
G.A. Baghdady
gabdrabboh65@yahoo.com
1
Department of Horticulture , Faculty of Agriculture, Al-Azhar University, Nasr city ,Cairo, Egypt.
This study was an attempt to develop an in vitro protocol for propagation date palm(Phoenix dactylifera L.) Zaghlol cv via direct shoot regeneration system. Combinations between plant growth regulators were used in order to achieve this goal. Shoot tips were cultured on MS medium supplemented with cytokinins namely BA or KIN at different concentrations either individually or in combinations with NAA at 0.0, 1.0, 2.0 and 4.0 mg /L in the presence of 1.0 mg/ L NOA. Results revealed that MS medium supplemented with BA or KIN at 1.0 mg /Lin combination with NAA at 1.0 mg/ L was the best for bud formation from shoot tip after 8 weeks of culturing at initiation stage. Sub-culturing the formed buds on solid MS medium supplemented with 1.0 mg/ L of BA and KIN gave the maximum number of shoots with appropriate length at multiplication stage. MS medium supplemented with 10 mg/ L of GA3 caused the maximum shoot length at elongation stage compared with control and other treatments. Moreover, maximum rooting percentage, root number and root length were obtained as MS medium supplemented with 0.5 mg/L IBA and 1.0 mg/L NAA was used.
https://assjm.journals.ekb.eg/article_65122_943079bc07c41016859e2da0948c83f1.pdf
in Vitro
shoot tips
growth regulators
direct pathway
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
137
144
10.21608/assjm.2018.65123
65123
Original Article
Salinity Stress Responses in Some Grape Rootstocks.
H. F. Abdel Aziz
hosny_fathy86@azhar.edu.eg
1
Department of Horticulture, Faculty of Agriculture, Al-Azhar University, Nasr city, Cairo, Egypt.
The study was conducted between 2014 and 2016 to monitor the effect of high level concentrations of NaCl on the micropropagation of some grape rootstocks Salt creek, Dog ridge, Richter and Freedom. The genetic analysis of molecular markers was performed using five primers of Inter Simple Sequence Repeat (ISSR). The five ISSR primers produced a total number of amplified bands ranged from 2 to 7 fragments. The highest number of fragments was 7 bands for 17898-B primer. While HB-15 primer generated the lowest number of amplicons (2 bands). The percentage of polymorphism revealed by the different primers ranged from 33.33 to 83.33 % with an average of 54.29 %. In our analysis we were able to identify and evaluate the grape genetic diversity and to study the phylogenetic relationships between cultivars. Also, the soluble proteins of leaves from four tested grape rootstocks were separated by slab gel electrophoresis into 12 protein bands, but only 2 to 5 bands were found in each cultivar. The peroxidase patterns exhibited a total number of 7 bands in each cultivar three of them were monomorphic bands (Rf = 0.077 – 0.138 – 0.757) in rootstock and under salinity stress. The generic variations detected among closely related genotypes indicate the efficiency of protein and isozyme markers for evaluating and constructing genetic linkage maps and the biochemical genetic diversity of these cultivars.
https://assjm.journals.ekb.eg/article_65123_526e0f83f26417ff2cb3355b68d7b025.pdf
salinity stress
Grape Rootstocks
ISSR
NaCl
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
145
146
10.21608/assjm.2018.65124
65124
Original Article
Genes associated with lemon floral transition and flower development during floral inductive water deficits (Abstract)
Jinzhi Zhang
jinzhizhang@mail.hzau.edu.cn
1
College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
Water deficit is a key factor to induce flowering in many woody plants, but reports on the molecular mechanisms underlying floral induction by water deficit are scarce. Here, we analyzed the morphology, cytology, and different hormone levels of lemon buds during floral inductive water deficits. Higher levels of ABA were observed, and the initiation of floral bud differentiation was examined by paraffin sections analysis. A total of 1638 differentially expressed genes (DEGs) were identified by RNA sequencing. DEGs were related to flowering, hormone biosynthesis, or metabolism. The expression of some DEGs was associated with floral induction under different hormone treatments. Four genes from the phosphatidylethanolamine-binding protein family were further investigated. Ectopic expression of these genes in Arabidopsis changed the flowering time of transgenic plants. Furthermore, the 5′ flanking region of these genes was also isolated and sequence analysis revealed the presence of several putative cis-regulatory elements, including basic elements and hormone regulation elements. The spatial and temporal expression patterns of these promoters were investigated under water deficit treatment. Based on these findings, we propose a model for citrus flowering under water deficit conditions, which will enable us to better understand the mechanism of water deficit–regulated flowering in citrus.
https://assjm.journals.ekb.eg/article_65124_374caa0ad8fa093b5c3d5eed57a50ad8.pdf
Lemon floral
transition
flower development
RNA
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
147
148
10.21608/assjm.2018.65125
65125
Original Article
Duplication of an upstream silencer of FZP significantly increases grain yield in rice (Abstract)
Yongzhong Xing
yzxing@mail.hzau.edu.cn
1
National Key Lab of Crop Genetic improvement, Huahzong Agricultural University, 430070, Wuhan China
Transcriptional silencer and copy number variants (CNVs) are associated with gene expression. However, their roles in generating phenotypes have not been well studied. In this study, we identified a rice quantitative trait locus (QTL), SGDP7 (Small Grain and Dense Panicle 7). SGDP7 is identical to FZP (FRIZZLE PANICLE), which represses the formation of axillary meristems. An 18-bp fragment, named CNV-18bp, was inserted ~5.3 kb upstream of FZP, thus resulting in a tandem duplication in Chuan 7. The CNV-18bp duplication repressed FZP expression, prolonged the panicle branching period and increased grain yield by more than 15% by substantially increasing the number of spikelets per panicle (SPP) and slightly decreasing 1000-grain weight (TGW). The transcription repressor OsBZR1 binds the CGTG motifs in CNV-18bp and represses FZP expression, indicating that CNV-18bp is the upstream silencer of FZP. These findings showed that CNVs of the silencer coordinate a trade-off between SPP and TGW by fine-tuning FZP expression, and balancing the trade-off would enhance yield potential.
https://assjm.journals.ekb.eg/article_65125_68f670da9bd34d9a97e62ea49c7ca95a.pdf
China
rice grain yield
rice QTL
FZP
SGDP7
tandem duplication
transcription repressor
upstream silencer
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
149
150
10.21608/assjm.2018.65126
65126
Original Article
Physiological basis and molecular mechanism of cold resistance enhanced by molybdenum application in winter wheat (Abstract)
Xuecheng Sun
sxccn@mail.hzau.edu.cn
1
College of Resource and Environment, Huazhong Agricultural University, Wuhan, China
Molybdenum (Mo) is an essential element for plant growth and development. Mo itself seems to gain biological function until it is complexed by Mo-containing enzymes. Mo application increase the yield of wheat by 20–30% in Mo-deficiency areas and enhanced the cold-resistance of winter wheat. In order to improve our understanding of the molecular mechanisms of cold resistance arising from application of Mo in winter wheat, effects of molybdenum on Mo-enzymes, hormones, antioxidative enzymes, expression of cold-responsive (COR) genes and quantitative proteomics, composition of thylakoids and photosynthetic parameters were investigated. The results showed Mo increased the Mo-containing enzymes such as NR, AO and XDH. Further evidence showed that Mo might regulate the expression of ABA-dependent COR genes through the pathway: Mo→AO→ABA→bZIP→ABA-dependent COR genes in winter wheat. In addition, most of differential proteins were located in membrane of thylakoids, and their functions were closely related to light reactions and dark reactions of photosynthesis. The results of COR gene expression and differential proteins were also supported by the results concerning composition of thylakoids and photosynthetic parameters. So a mechanism was initially put forward: molybdenum regulated the gene expression via Mo-enzymes, and then successively affected the protein expression, metabolic processes, physiological functions such as enhancing the photosynthesis, andcold resistance in winter wheat.
https://assjm.journals.ekb.eg/article_65126_311576e943369fa024b1e1902ddad4bb.pdf
Cold resistance
molybdenum
winter wheat
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
151
152
10.21608/assjm.2018.65127
65127
Original Article
Recent advances in the regulation of citric acid metabolism in citrus fruit (Abstract)
Liu Yongzhong
liuyongzhong@mail.hzau.edu.cn
1
College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China
Regulation of citric acid metabolism during fruit ripening is a major concern of better quality fruit production. Moreover, significant involvement of citric acid in determining organoleptic fruit quality attributes, fruit storage performance and synthesis of several secondary metabolites, has led to exponential increase in research efforts in last two decades. As a result, noteworthy information on citric acid metabolism is available to the worldwide citrus industry. Recent researches indicate the relationship among citric acid biosynthesis, transportation, storage and utilization. Among citrate metabolic processes, citrate storage in vacuole and its subsequent catabolism in cytosol play an important role in regulation of citrate metabolism. This review is anticipated to summarize recent research progresses towards citrate metabolism to a scientific community. We provide an overview of citrate metabolism, postharvest physiology of citrate metabolism and influence of agro-climatic factors in citrus fruits, and highlight recent advances. Moreover, this is the first review which shows a comprehensive citrate metabolic model in citrus fruit juice sacs and it will be hallmark for citrus as well as other fruit trees in future. In addition, this review facilitates the practical implication of citrate regulatory model in regulating the fruit acidity in other non-climacteric fruits.
https://assjm.journals.ekb.eg/article_65127_1c93ce3c0b420da02658e8fd066d39ae.pdf
Citric acid metabolism
citrus fruit
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
153
154
10.21608/assjm.2018.65128
65128
Original Article
Omics provide insights to understand cotton responsive to Verticillium dahliae and application (Abstract)
Longfu Zhu
lfzhu@mail.hzau.edu.cn
1
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
Due to no high resistant germplasm in upland cotton (Gossypium hirsutum), Verticillium wilt has become the most serious disease for cotton production in China. Through whole genome gene expression analysis by RNA sequence, we found the expression level of lignin synthesis-related genes and the activity of corresponding enzymes induced more quickly in resistant cultivar ‘7124’ (G. barbasense) [1]. Recently, an ethylene response-related factor, GbERF1-like, from '7124' was proved as a positive regulator in lignin synthesis and contributed substantially to resistance to V. dahliae [2]. And suppression the expression level of a cotton P450 gene, SILENCE-INDUCED STEM NECROSIS (SSN), causes a lesion mimic phenotype in cotton. Further study shows that SSN silencing causes an imbalance in LOX (lipoxygenase) expression and excessive hydroperoxide fatty acid accumulation. We also show that an unknown oxylipin-derived factor is a putative mobile signal required for systemic cell death [3]. Complex phytohormones interaction was also found involving in cotton responsive to V. dahliae [4]. BR and JA signal pathways play essential roles in interaction of cotton and V. dahliae [4, 5]. Furthermore, we identify the key data from genomics and proteomics with a data-mining strategy accompanied by VIGS and heterologous expression [6]. GbWRKY1 is one of the key candidate genes and has been proved with as a critical regulator mediating the plant defense-to-development transition during V. dahliae infection by activating JAZ1 expression [7]. Overall, our study provides highlights to understand the mechanism of cotton resistance to V. dahliae at molecular level.
https://assjm.journals.ekb.eg/article_65128_56932009db43927e1b8b4de8a055ee67.pdf
Omics
Cotton
verticillium dahliae
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
155
156
10.21608/assjm.2018.65130
65130
Original Article
High-density genetic map construction and identification of quantitative trait loci controlling leaf abscission in citrus (Abstract)
Chungen Hu
chungen@mail.hzau.edu.cn
1
College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
We constructed a high-density genetic map of citrus using simple sequence repeat and single-nucleotide polymorphism markers evenly distributed across the genome. The sex-averaged linkage map contains 4163 markers with an average distance of 1.12 cM between loci. The female clementine mandarin linkage map contains 1478 markers with a length of 1093.90 cM. A dense genetic map of male parent Poncirus trifoliata was also constructed, with a length of 1227.03 cM and 2976 markers. Collinearity between physical and genetic distances of all markers in nine linkage groups indicated the high quality of the genetic map. The linear order of common markers was highly conserved between clementine mandarin and P. trifoliata. Poncirus has deciduous leaves, representing a distinct phenotype from evergreen true citrus trees. Based on this high-density integrated citrus genetic map and two years of deciduous phenotype analysis, two potential quantitative trait loci (QTLs) were identified in linkage groups 1 and 8, and 137 candidate genes were identified from these regions based on integrative analysis of resequencing and transcriptome analysis. The high-density citrus genetic map will greatly facilitate QTL mapping and genome studies, and successful localization of the deciduous trait is valuable for understanding the underlying mechanism and improving citrus breeding.
https://assjm.journals.ekb.eg/article_65130_0e7417fa40cf522f45b2c513c8df8f5d.pdf
QTL mapping
leaf abscission
citrus
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
157
166
10.21608/assjm.2018.65131
65131
Original Article
Genetics studies on inheritance of stripe rust resistance in wheat.
Makhlouf Bekhit
makhlouf.bakhit@fagr.bu.edu.eg
1
Faculty of Agriculture at Moshtohor, Benha University
This work was carried out to study the response of four Egyptian bread wheat cultivars i.e Giza 168, Giza 171, Sids 12 and Sids 13, to yellow rust disease and its effect on grain yield components under field condition at Nobaria Agriculture Research Station, during two growing seasons , (2015/16 – 2016/17). Results showed that there was a significant difference among the tested cultivars from the most of the studied yield parameters. Giza 171 had the highest significant values of days of heading plant height, grain yield, number of grain/spike and 1000 grain yield in the two seasons. On the other hand according to disease assessment Sids 12 had the highest significant values of final rust severity (FRS), rate of disease increase (r-value) and area under disease progress curves (AUDPC) in the two seasons. Inter-simple sequence repeat (ISSR) marker was used to measure the genetic diversity between one Stripe rust resistant wheat cultivar (YR7), the four local cultivated varieties and the F1 cross progeny. Ten ISSR primers were used, a total of 109 bands were amplified, among which 41 (37%) bands were polymorphic. The polymorphic bands amplified by each primer ranged from 2 to 8, with an average of 4.1. The genetic similarity ranged from 88% to 96%.
https://assjm.journals.ekb.eg/article_65131_9e5d139805eb9d0cd5b7f177ffd76fa5.pdf
Strip rust
Wheat
ISSR
PCR
Disease
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
167
176
10.21608/assjm.2018.65134
65134
Original Article
In vitro, induction of salt tolerant potato (solanum tuberosum l.) Plants with gamma irradiation and characterization of genetic variations through sds-page and issr-pcr analysis
Dina ELHetawy
dodecats.1992@gmail.com
1
Department of Genetics and genetic engineering, Faculty of Agriculture, University of Benha, Egypt.
Salt tolerant mutants of potato (Solanum tuberosum L. ‘Sponta’) were obtained via gamma irradiation. bud explants of two strains of potato (Sponta and lady rosetta were treated with various dosages of gamma irradiation, and the clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 30, 60, 90 and 120 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using ISSR technique, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants were genetically 27.5% different from the control plants. The greatest difference encountered between the control and mutants was 47%, which was detected in mutant plants produced by 20 or 30 Gy gamma irradiation and regenerated in selection medium containing 100 mM NaCl.
https://assjm.journals.ekb.eg/article_65134_f00073a90ab96f5a0b7aeed33f19be00.pdf
gamma radiation
in vitro mutagenesis
Mutation breeding
potato
ISSR
salt stress
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
177
186
10.21608/assjm.2018.65136
65136
Original Article
Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in Stevia rebaudiana (Bertoni).
Makhlouf Bekhit
makhlouf.bakhit@fagr.bu.edu.eg
1
Faculty of Agriculture at Moshtohor, Benha University
Chromosome number was investigated in a number of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and different stages of meiosis. All stevia strains were found to had 2n = 22. The number of bivalents at diakinesis was 11 for all the studied strains. Analysis of SDS-PAGE of soluble protein revealed complete similarity (100%) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band. Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR). A total of 231 clear bands were generated, out of which 34 (14.72 %) were unique bands. The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer.
https://assjm.journals.ekb.eg/article_65136_818df22b71de38105f06912f41c9c8c9.pdf
Chromosomes
Stevia
bivalents
SDS-PAG Eelectrophoresis
ISSR
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
187
202
10.21608/assjm.2018.65137
65137
Original Article
Effect of foliar spray with lithovit and amino acids on growth, bioconstituents, anatomical and yield features of soybean plant
M. Abd El-Aal
mohamed.abdelal@fagr.bu.edu.eg
1
Agricultural Botany Department, Faculty of Agriculture, Benha University, Moshtohor, Toukh, Qalyoubia, 13736, Egypt
A field study was carried out at the Experimental Farm Station of Faculty of Agriculture, Moshtohor, Benha University, Kalubia Governorate, Egypt, during the successive summer seasons of 2016 and 2017. This experiment was conducted to study the effect of foliar application with lithovit at 250 and 500 mg/l and amino acids at 2 and 4 ml/l on some growth aspects, biochemical constituents, anatomical and yield characteristics of soybean plants cv. Giza 111. The obtained results indicated that foliar application with lithovit at a rate of 500 mg/ L and amino acids at a rate of 4 ml/l caused significant increases in all vegetative growth characteristics expressed as plant height, stem diameter, number of leaves/ plant, total branches/plant, total leaf area/ plant, shoots fresh and dry weights g/plant and specific leaf weight/plant as compared with the control treatment. Data also indicated that the application of lithovit at a rate of 500 mg/l and amino acids at 4 ml/l treatments gave the highest values of photosynthetic pigments (chlorophyll a, b and carotenoids), phytohormones concentration and leaf chemical compositions (N, P, K, Ca, Mg %, Fe ppm, total carbohydrates, crude protein %) as well as enhancing leaf and stem anatomical features of soybean plant. Also, the obtained results showed that foliar application with lithovit at 500 mg/l and amino acids at 4 ml/l treatments caused significant increases in flowering and yield characteristics i.e., number of flowers/plant, number of setted pods/plant, pods yield/plant, number of seeds/pod, weight of seeds/pod, 100 seeds weight, weight of seeds/plant (g) in the two growing seasons as well as seeds oil percentage and unsaturated fatty acids % as compared with the control. Generally, it could be concluded that foliar application with growth stimulators as lithovit at 500 mg/l and amino acids at 4 ml/l could be recommend in soybean cultivation for improving its growth, productivity and quality.
https://assjm.journals.ekb.eg/article_65137_dc81a952fa5d603fa6afd26b7a60edcb.pdf
Soybean
Lithovit
Amino acids
Growth
chemical compositions
anatomy
yield and fatty acids
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
203
206
10.21608/assjm.2018.65138
65138
Original Article
In Vitro Antibacterial Effects of Pomegranate Fruit Peel Infusion Against Two Diarrheagenic Bacteria
Mayadah Ali
mayyada.hajali@gmail.com
1
Department of Plant pathology, Faculty of agriculture, Cairo University, Cairo, Egypt.
Pomegranate, which is known as Rumman in Arab region, has been used in traditional medicine in many countries for its beneficial health effects to treatment various illnesses as dysentery, diarrhea, acidosis and many other diseases. Water extract of pomegranate has been used to treatment diarrhea in Syria for long time, and dried peels are kept in almost every house in Damascus for this purpose. However, the contents of water extract of local pomegranate peel, which is used in traditional method to cure diarrhea, and the in vitro antibacterial activity are not investigated.
In the current study we tried to investigate the total phenolic contents as indicator of beneficial compounds in the water extract of pomegranate peels obtained from the two varieties (sweet and sour pomegranate), and the antibacterial activity of these extracts against two foodborne pathogenic bacteria namely Bacillus cereus and Salmonella typhi, which are responsible for diarrhea syndromes.
https://assjm.journals.ekb.eg/article_65138_4b72b38335accd8de4d20f5f02b87b93.pdf
pomegranate
Water Extract
Phenolic Compounds Bacillus cereus
Salmonella typhi. Minimal Bactericide Concentration (MBC)
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
207
214
10.21608/assjm.2018.65139
65139
Original Article
Effect of silicon and algae extract foliar application on growth and early yield of globe artichoke plants.
H. Abd El-Dayem
hosny_abdeldayem@yahoo.com
1
Plant Botany Department, Faculty of Agriculture, Benha University, Egypt
Field experiments were carried out in Vegetables Research Farm of Kaha, Kalyoubia, Horticulture Research Institute, A.R.C., Egypt to investigate the effect of sprayingwith silicon (1000 and 2000 ppm SiO2) or algae extract (2 ml/L and 4 ml/L) sprayed twice at 45 and 60 days after planting on the growth, early yield, total yield and chemical components on globe artichoke plants of French cultivar (Hyrious) which were grown under drip irrigation during the two successive seasons of 2015/2016 and 2016/2017. The obtained results showed that globe artichoke plants were sprayed with silicon (2000 ppm SiO2) or algae extract (4 ml/L) recorded the highest significant increase in all studied growth characters (i.e., plant height, numbers of leaves per plant, total leaves area per plant, fresh and dry weight of shoot). As well as significant increase in the yield parameters (i.e., head diameter, fresh head weight, head length, fresh and dry weight of receptacle) also, the same treatments recorded the highest values of early and total yield per feddan and significant increase chlorophyll a, b , total chlorophyll , in nitrogen, phosphorus and potassium concentrations as well as total sugar and total amino acid concentrations followed by plants sprayed with silicon (1000 ppm SiO2) or algae extract (2 ml/L) in the two successive seasons when compared with untreated plants.
Conclusively, from that obtained data in this study, it can be recommend the use silicon (2000 ppm SiO2) or algae extract (4ml/L)as foliar spray on globe artichoke plants for improve their growth, early and total yield.
https://assjm.journals.ekb.eg/article_65139_027fde15c1e58953236676f1ac5e26f1.pdf
Globe artichoke
Silicon (SiO2)
Algae extract
and Stimulation growth
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
215
216
10.21608/assjm.2018.65524
65524
Original Article
Microbial arsenic oxidation and chemotaxis (Abstract)
Gejiao Wang
gejiao@mail.hzau.edu.cn
1
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China
We isolated a highly As(III) resistant [minimal inhibitory concentration (MIC) = 25 mM] and As(III)-oxidizing bacterium A. tumefaciens GW4 from As-enriched groundwater sediments. Unlike most of the heterotrophic As(III)-oxidizing bacteria using As(III) oxidation as a detoxification process, the As(III) oxidation of strain GW4 enhanced the bacterial growth, and the strain showed positive chemotaxis toward As(III) towards 0.5-2 mM As(III). Genomic analyses revealed a putative chemoreceptor-encoding gene, mcp, located in the arsenic gene island and having a predicted promoter binding site for the As(III) oxidation regulator AioR. Expression of mcp and other chemotaxis related genes (cheA, cheY2 and fliG) was inducible by As(III), but not in the aioR mutant. Using capillary assays and intrinsic tryptophan fluorescence spectra analysis, Mcp was confirmed to be responsible for chemotaxis towards As(III) and to bind As(III) (but not As(V) nor phosphate) as part of the sensing mechanism. A bacterial one-hybrid system technique and electrophoretic mobility shift assays showed that AioR interacts with the mcp regulatory region in vivo and in vitro, and the precise AioR binding site was confirmed using DNase I foot-printing. Taken together, these results indicate that this Mcp is responsible for the chemotactic response towards As(III) and is regulated by AioR. Additionally, disrupting the mcp gene affected bacterial As(III) oxidation and growth, inferring that Mcp exerts functional connection between As(III) oxidation and As(III) chemotaxis.
https://assjm.journals.ekb.eg/article_65524_d67f5e856701f5248239600acb2bedcb.pdf
Chemotaxis
microbial arsenic oxidation
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
217
226
10.21608/assjm.2018.65525
65525
Original Article
Microbiological and physicochemical evaluation of River Nile (Rosetta branch)
Rasha El-Meihy
rashaelmehy@fagr.bu.edu.eg
1
Agricultural Microbiology Department, Faculty of Agriculture, Benha University, Egypt.
This study was carried out during period extended from January 2013 to December 2013 to evaluate the microbiological and physicochemical characteristics of Rosetta branch water, River Nile. The examination includes eight locations along Rosetta branch. Temperature values were ranged between 17.6 to 31.0ºC while, pH values ranged between 7.16 to 7.98 during four seasons. Moreover, turbidity values were ranged between 1.90 to 33.7 NTU during all seasons. Regarding dissolved oxygen (DO) value, the lowest (3.1 mg/l) and the highest (8.0 mg/l) values were recorded during autumn and winter, respectively. Moreover, the highest (26.6 mg/l) and the lowest (3.30 mg/l) values of biological oxygen demand (BOD) were recorded during winter and autumn, respectively. Chemical oxygen demand (COD) values were ranged between 7.4 to 42.6 mg/l which recorded during winter season. Additionally, ammonia values were ranged between 0.22 to 7.70 mg/l during all four seasons. Nitrite values ranged between 0.002 to 0.071 mg/l during all four seasons. The lowest nitrate value (0.10 mg/l) and the highest value (1.02 mg/l) were recorded during summer and winter, respectively. Respecting the monthly changes in microbial counts of Rosetta branch, average log no. of total coliform was ranged between 1.84 to 6.88 CFU/100ml, the highest log no. in most locations were recorded during July. While, average log no. of fecal coliform was ranged between 1.30 to 6.5CFU/100ml during all months. Moreover, the highest counts of log no. was (5.96 CFU/100ml) and the lowest log no. was (0.30 CFU/100ml) of E. coli were recorded during August. Additionally, the highest log no. of fecal streptococci (4.85 CFU/100ml) was recorded during July.
https://assjm.journals.ekb.eg/article_65525_101ab6d17979e4695749c634fef04ee8.pdf
Rosetta Branch
microbiological
physicochemical
River Nile
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
272
242
10.21608/assjm.2018.185525
185525
Original Article
Removal of heavy metal ions from some wastewater by using different agricultural wastes
E.A Dwidar
enaskh10@hotmail.com
1
Fac. of Agric. Benha Univ. Egypt
Industrial wastewaters are deposed into the River Nile without any treatment. High concentrations of some heavy metals i-e Ni+2, Cu+2, Zn+2, CO+2 and Cd+2, promate the growth of gelatinous masses and hence cause industrial and agriculture problems. Polluted water may cause many health problems such as liver and kidney diseases. The aim of the present study is an attempt to removal heavy metals in industrial wastewater. Also, evaluation of some agricultural wastes such as orange peels, banana peels and leaves of date trees as biosorbent to remove the heavy metals which presenting from wastewater such as nickel (Ni+2), copper (Cu+2), zinc (Zn+2), lead (pb+2), cobalt (CO+2) and cadmium (Cd+2) by using prepared active carbons from the above-mentioned materials of different agricultural wastes in batch adsorption process. Proximate, chemical components of lignocellulosic wastes under investigation were determined. Also, physical and chemical preparation of wastewater and the produced active carbons from different agro-wastes were evaluated. On the other hand, the activated carbons from different agro-wastes were used as biosorbents for removal of some above-mentioned heavy metals before deposing in wastewater.
https://assjm.journals.ekb.eg/article_185525_f96a60bb9a8b22b9723271d96f7f21f9.pdf
Agriculture wastes
activated carbons
Heavy metals
Adsorption
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
243
252
10.21608/assjm.2018.185528
185528
Original Article
Evaluation of biological activities for salt-tolerant plant growth promoting rhizobacteria using different microbial carriers
A. A. Salem
ahmed.nayl@fagr.bu.edu.eg
1
Agric. Microbiol, Botany Dept., Fac. of Agric. at Moshtohor, Benha Univ.
Plant growth promoting rhizobacteria (PGPR) play an important role for improving plant growth and increasing productivity especially under stress condition. Fifty rhizobacterial isolates were isolated from the rhizosphere of the wheat plants. The rhizobacterial isolates were screened to select the most salt tolerant isolates. Then, the more tolerant isolates were evaluated for plant growth promoting activities such as ammonia, HCN, siderophores, nitrogenase, indole acetic acid (IAA) and gibberellins (GB) production as well as, root colonization ability, phosphate and potassium solubilization. The obtained data showed that, rhizobacterial isolate number (40) exhibited high records for most of PGPR activitiesand identified asPaenibacillus polymyxaMG309677.1using 16S rRNA gene sequencing techniques with 99% similarity. One of the problems which face production of microbial preparations for agriculture use is the maintenance of bacterial viability. In this respect, immobilization of P. Polymyxa MG309677.1 on three different carriers (peatmoss, compost and sawdust) was studied. Peatmoss and compost were used singly or combined with sawdust at two levels (50% and 75% sawdust).Bacterial populations, dehydrogenase activity, pH values and moisture contents were determined monthly up to six months of storage. Resultsrevealed that, using of peat moss either singly or combined with sawdust (50:50%) as a carrier gave the highest survival and population records forP. polymyxaMG309677.1even the end of storage period (180) days.
https://assjm.journals.ekb.eg/article_185528_d1c167df71bfb155d5c02ddd828e5bc2.pdf
ompost
peat moss
Carriers
Paenibacillus polymyxa
survival and biological activities
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
253
262
10.21608/assjm.2018.185533
185533
Original Article
Mechanism of antimicrobial activity and antioxidant activities of the essential oil and the methanolic extract of carum montanum from Algeria
MERIEM EL KOLLI
elkollim@yahoo.fr
1
Laboratory of Natural Biological Resources, University of Sétif 1, Sétif, Algeria.
The methanolic extract (ME) of C. montanum obtained by a hydo-alcoholic maceration and its polyphenol content was evaluated by Folin-Ciocalteu method. This extract and C. montanum essential oil (EO)were screened for antimicrobial activity against 21 microbial strains by agar diffusion method. MICs of the EO were determined by the broth micro dilution method. The action mechanism of EO was determined on the susceptible strains by the time kill assay and the lysis experience. Antioxidant properties were studied by both free DPPH radical scavenging and reducing power techniques. The TPC in the ME showed a high level of 101.50 ± 5.33 mg GAE /mg. B. cereus was the most sensitive strain with MIC of 55.5 µg/ml , then K. pneumoniae (111 µg/ml). A remarkable decrease in a survival rate as well as in the absorbance at 260 nm were recorded, which suggest that the cytoplasm membrane is one of the targets of the EO. Antioxidant effects concentration were dependent and IC50 values were 1.09 ± 0.37 µg/ml for the EO and 65.04 ± 0.00 µg/ml for the ME by DPPH method and a reducing power dose-dependent. In conclusion, C. montanum extracts showed potent which could be exploited in the food industry for food preservation.
https://assjm.journals.ekb.eg/article_185533_b74f886e3865b5540450e44f12412308.pdf
C. montanum
Apiaceae
Essential oils
antimicrobial activity
Antioxidant activity
Reducing power
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
263
264
10.21608/assjm.2018.185534
185534
Original Article
Changes of dissolved organic matter derived from rape straw by Selenium pretreatment improve the inhibition of Sclerotinia sclerotiorum growth (Abstract)
Xiaohu Zhao
xhzhao@mail.hzau.edu.cn
1
College of Resources and Environment, Huazhong Agricultural University, Wuhan, China
In this study, dissolved organic matter (DOM) was extracted from rape straw pretreated with Se in soil to examine the inhibitory effect on the growth of Sclerotinia sclerotiorum by poisoned culture medium technique. The results revealed that DOM in culture medium inhibited the growth of S. sclerotiorum significantly, while the DOM derived from rape straw pretreated with Se achieved the better inhibitory effect. To confirm that Se pretreatment in soil caused changes in DOM derived from rape straw which increased antifungal effect on S. sclerotiorum, a two-year laboratory pot culture experiment with four levels of Se (control, 0.1,0.5, 1.0 mg Se kg-1 soil) was conducted and the changes of the component and chemical structure in DOM were investigated using UV-visible spectroscopy, fluorescent spectroscopy and FTIR spectroscopy. Results of spectrograms showed that application of Se in soil altered the DOM properties, such as getting more irreplacable aromatic structure in substituent groups of DOM, achieving less conjugated systems, and gaining more alcohol, phenol, amine, amino acid salt and alkyne in DOM, and all the changes of DOM chemical composition improved the inhibition of S. sclerotiorum growth. The results of this study not only provide agricultural environmental safety information for more effective utilization of rape straw , but also light another approach of Se application in agricultural science.
https://assjm.journals.ekb.eg/article_185534_6972695d688b5d7083323fd296339bf4.pdf
selenium
rape straw
dissolved organic matter (DOM)
composition
inhibition
Sclerotinia sclerotiorum
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
265
274
10.21608/assjm.2018.214382
214382
Original Article
Isolation and Characterization of Zinc Tolerant Bacteria from Contaminated Sediments and Soils in Egypt
Rasha El-Meihy
rashaelmehy@fagr.bu.edu.eg
1
Agricultural Microbiology Department, Faculty of Agriculture, Benha University, Egypt.
A total of 71 zinc tolerant bacteria were ,isolated from Four heavy metals contaminatedsediments and soils in Egypt, three sediment samples (Upstream, midstream, downstream Al-Rahawy drain, Giza Governorate) and two agricultural soil samples (Al-Gabal Al-Asfar region and Kafr-Ilwan village, Qalubia Governorate). The minimum inhibitory concentration (MIC) and maximum tolerance concentration (MTC) under zinc (Zn+2) concentrations ranged between 500-4500 mg/l. The most zinc tolerant isolate UR45 with MIC 4500 mg/l and MTC 4000 mg/l was identified by partial sequence of 16S rRNA genes as Alcaligenes faecalis MG257493.1 (UR45). Tolerance of A. faecalisto cadmium (Cd2+), copper (Cu2+) and lead (Pb2+) under different concentrations (1000-4500 mg/l) was evaluated. Growth of the strain and its biosorption activities (metal uptake, metal residual, metal biosorption) at 1000 mg/l of Zn2+, Cd2+, Cu2+ and Pb2+ under different pH values (5-9) was studied. pH 8 was optimum for growth in media supplemented with Zn2+ or Cu2+. Whereas, the biosorption potentials were differed according to the examined metal. Finally, enzymatic (catalase, peroxidase, polyphenol oxidase) and non-enzymatic (inhibition 2,2-DiPhenyl-2-Picrylhydrazyl hydrate (DPPH)) antioxidant activities of the A. faecalis MG257493.1 in presence of four heavy metals individually or in mixture at 1000 and 1500 mg/l were estimated. The obtained data showed that most antioxidant activities of the strain was increased with the increasing of heavy metals concentrations up to 1500 mg/l.
https://assjm.journals.ekb.eg/article_214382_6ed46fc6f4cc83bea51b50b588314204.pdf
Heavy metals
zinc
Alcaligenes faecalis
Biosorption
Antioxidant activities
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
275
284
10.21608/assjm.2018.214383
214383
Original Article
Chemicals and Biological Study on Tortilla Chips
Omar A. El-Shayeb
omar.elshayeb@pepsico.com
1
*Agric. Biochemistry Dept., Fac. of Agric., Moshtohor, Benha Univ.
The present study was designed to determine the chemical and biological effects for flavors added to tortilla chips (cheese onion, tandoori chicken, sweet pepper, and chili and lemon) compared with unflavored tortilla chips (homemade) as control. Rats used in the biological study, were fed for 60 days on the tortilla chips with the different flavors and the control. The effect of these flavors on the liver and Kidney function were recorded. Blood samples were collected by withdrawing in zero time 60 day from vein plexus eye and the serum was obtained. ALT, AST, alkaline phosphatase, albumin, total protein, creatinine and uric acid were determined.
The chemical composition of tortilla chips flavored with (Cheese onion, sweet chili pepper, chili and lemon or Tandoori Chicken compared with unflavored tortilla chips. The data revealed that the crude protein ranged between 6.25-6.97%, total lipid 22.30-25.49%, total carbohydrates 59.57-61.17%, crude fiber 12.81-17.44%, Moisture 1.20-1.79%, Starch 50.25-57.04% and total ash 1.05-2.94% for the above mentioned flavored chips, respectively. Levels of marker enzyme alanine transaminase (ALT) aspartate transaminase (AST), alkaline phosphatase (ALP) were increased on the other hand serum total protein and serum albumin were decreased. The results obtained showed that the flavored tortilla chips had badly affected the examined blood serum compared with the unflavored tortilla chips (homemade).
https://assjm.journals.ekb.eg/article_214383_d41d8cd98f00b204e9800998ecf8427e.pdf
tortilla chips
Flavors
Cheese onion
sweet chili pepper
chili and lemon
Tandoori Chicken
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
287
298
10.21608/assjm.2018.214385
214385
Original Article
Improve the quality of English Cake by using of some Enzymes
ashraf sharoba
ashraf_sharoba@yahoo.com
1
Department of Food Technology, Faculty of Agriculture, Benha University, Egypt.
Bakeries occupy an important position in human nutrition locally and globally. Given the gap between production and consumption, governments are importing wheat from different countries. Ukrainian wheat is therefore the most widely used wheat in Egypt and Iraq. In order to match their characteristics with the local concessions, whether Egyptian or Iraqi.The study investigated the use of Ukrainian wheat in the production of 72% flour for the manufacture of English cake. The study also included the evaluation of some enzyme enhancers, which included α- amylase (0.3%) and glucose oxidase (0.1%). The study also examined the characteristics of the English cake in terms of (chemical composition, gluten, rheology, textures, staling, microbiology, sensory) during the storage period of 12 days at 25 ± 1 ° C. The results showed a clear and noticeable improvement of all technological characteristics under study, the most important of which was the increase of the validity period to reach 12 days for the English cake compared to the sample (control). Therefore, the study recommends the use of the following α-amylase, commercial glucose oxidase in the manufacture of some bakery products.
https://assjm.journals.ekb.eg/article_214385_948b6955017e198e29aece9a64cdf163.pdf
Ukrainian wheat, α- amylase, Rheological, chemical composition
microbiological examination, English cake
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
299
314
10.21608/assjm.2018.214390
214390
Original Article
Use of some hydrocolloids to improve the quality of Iraqi bread (Simon) product
ashraf sharoba
ashraf_sharoba@yahoo.com
1
Department of Food Technology, Faculty of Agriculture, Benha University, Egypt.
In this study, the most widely Russian wheat was used in Iraqi which similar to the characteristics of most of the cultivated Iraqi cultivars for use in this study. The chemical composition, rheological properties were estimated using modern and old technical. The aim of this work to study the effect of some hydrocolloids to improve the characteristics of Iraqi bread (Simon). Conduct a survey of the most important hydrocolloids used in bakery processing (pectin, carrageenan, sodium alginate, xanthan gum, guar gum and carboxy methylcellulose) to choose the best and add it to some bakery products. The best percentage of selected hydrocolloids was chosen according to the previous study of the sensory evaluation. Use of the hydrocolloids (xanthan gum, carrageenan and guar gum) and choose the best ratios for the hydrochlorides mentioned 0.75%, for carrageenan and guar gum, and 0.2%, for xanthan gum, based on sensory evaluation and economic assessment tests. Iraqi bread (Simon) was manufactured with the addition of some hydrocolloids in the above mentioned percentages. Quality attributes of iraq bread (Simon) were evaluation by chemical analysis, microbial safety tests, strength tests for product tests were carried out at the beginning of manufacturing and during storage at room temperature 25±2°C until the product begins to corrupt.
The obtained results showed that the using some hydrocolloids were improvement in the technological characteristics of iraqi bread product under study, the most important is to increase the shelf life to 8 days compared to products manufactured in the Egyptian and Iraqi market that the validity period approximately exceed three days. Therefore, recommend to use the following hydrochlorides (xanthan, carrageenan and guar gum) in the commercial range in the manufacture of product.
https://assjm.journals.ekb.eg/article_214390_d41d8cd98f00b204e9800998ecf8427e.pdf
Iraqi bread (Simon)
hydrocolloids
Chemical composition
Microbiological examination
improvement
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
315
324
10.21608/assjm.2018.214391
214391
Original Article
Utilization of Sprulina Algae to Improve the Nutritional Value of Kiwifruits and Cantaloupe Nectar Blends
H.E.M. Bahlol
hammam.bahlol@fagr.bu.edu.eg
1
Food Technology Department, Faculty of Agriculture, Benha University, Egypt.
In this study use the spirulina which is one of the blue-green algae rich in protein 61.57% and contains a high proportion of essential amino acids (38.81% of the protein) and a source of naturally rich in vitamins especially vitamin B complex such as vitamin B12 (193 μg/100 g) and folic acid (9.66 mg/100 g), which helps the growth and nutrition of the child brain, also rich in calcium and iron it containing (1043.625 and 338.765 mg/100 g, respectively) to protect against osteoporosis and blood diseases as well as a high percentage of natural fibers. So, the spirulina is useful and necessary for the growth of infants and very suitable for children, especially in the growth phase, the elderly and the visually appetite. It also, helps a lot in cases of general weakness, anemia and chronic constipation. Spirulina contain a selenium element (0.0488 mg/100 g) and many of the phytopigments such as chlorophyll and phycocyanin (1.472% and 14.18%), and those seen as a powerful antioxidant. Finally, spirulina called the ideal food for mankind and the World Health Organization considered its "super food" and the best food for the future because of its nutritional value is very high. In this study, products supported with spirulina, kiwifruits and cantaloupe were manufactured. Two types of vegetable and fruit juices, green and were used frequently in commercial manufacturing (cantaloupe - kiwi). Spirulina was added to them at different ratios (zero “control samples”, 2.5, 5, 7.5, 10, 12.5 and 15%) The nectar blends were well studied to determine the best percentage of addition and the chemical, natural were done and sensory properties of nectar blends supported by spirulina. The all nectar blends prepared and supported by spirulina were highly accepted. Therefore, it is recommended to use spirulina in the field of strengthening juices, especially the rich types of chlorophyll, anti-oxidant and beneficial to public health such as kiwifruits and cantaloupe.
https://assjm.journals.ekb.eg/article_214391_4fe7617afc805e8b00391e767162565c.pdf
Spirulina
Kiwi Nectar
Cantaloupe Nectar
Chemical composition
Nutritional Value
Sensory evaluation
physiochemical characteristics
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
325
336
10.21608/assjm.2018.214393
214393
Original Article
Evaluation of beef-burger in some Egyptian markets
Mahmoud Mohamed
mahmoudeldaf@yahoo.com
1
Food technology, Benha University
The current work aimed to assess effect of the market locality and season of sampling on the quality of beef-burger. The chemical, physical, microbiology and organoleptic properties of burger, were evaluated in winter (W) and summer (S) seasons for 6 samples (S1 -S6) from three different locations at both Cairo and Alexandria Governorates. The following chemical composites were moisture content in S4 (in both seasons), dry matter in S5 (in both seasons), crude protein in S3 (in both seasons), ether extract in S4 (S) and S3 (W), ash in S3 (S) and S4 (W), and carbohydrate in S6 (in both seasons). The following freshness tests were pH in S6 (S) and S4 (W), thiobarbituric acid in S1 (in both seasons), total volatile nitrogen in S6 (S) and S2 (W). The following heavy metals were maximal, zinc, copper and lead in S2, and Cadmium in S5 in (S), meanwhile, in winter the concentrations of zinc in S4, copper in S5, cadmium in S6 and Lead in S3 were the highest. Bacteriological load was above the Std. Specifications, aerobic plate count in S2 (in both seasons), Coliform count in S1 (S) and S5 (W), Staph. Count in S2 and S6 (in both seasons) beside S1 (S) and S5 (W), total mold count in S2, S4 and S5 (S) and S6 (W), and total yeasts count in S2 and S6 (in both seasons) beside S3 and S4 (S). Sensory evaluation of beef burger was of highest values, color in S4 (in both seasons), odor in S5 (in both seasons), taste inS2 (S) and S4 (W), hardness in S4 (S) and S5 (winter), juiciness in S1 (S) and S5 (winter), and overall acceptability in S4 (S) and S5 (W). In conclusion, the physio- chemical as well as the hygienic quality of beef burger under the Egyptian environmental conditions are greatly influenced with the markets locality as well as the season of the year.
https://assjm.journals.ekb.eg/article_214393_592b568d9d88d7f7b2adf2a9c7a6d221.pdf
Beef burger
Chemical composites
Freshness tests
heavy metal
Hygienic quality
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
337
346
10.21608/assjm.2018.214394
214394
Original Article
Influence of Ozonation and Storage of Wheat Grains on Chemical Composition and Rheological Properties
Ozonation of wheat grains is considered as a quick and easy process that could be realized during the storage. The ozone is in direct contact with the grain and may modify the properties of the wheat. In the present study the effect of ozonation local wheat (LW) and red Roman wheat (RRW) at 80 ppm with exposure time 30 and 60 min before storage for 3 and 6 months on chemical composition, rheological properties of wheat flourandcontentofamino acidswere carried out. The obtained results indicated that ozonated wheat grains did not significantly alter the chemical compositionof LW and RRW after ozonation. On the other hand stability and resistance of dough were increased with ozonation for 60 min in comparison with control sample. In general, the results indicated that increments in the amount of amino acids with increasing time of exposure to ozone gas.
https://assjm.journals.ekb.eg/article_214394_2d4386225f0fcf8fb7b16a6e1220b84d.pdf
Ozonation
Local wheat
Red Roman Wheat and amino acids
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
347
354
10.21608/assjm.2018.214395
214395
Original Article
Efficacy of wilting degree on physiochemical traits and sugar processing parameters of sugar beet roots postharvest
Under Egypt conditions sugar beet roots is processed in the factories during the period from the first week of February to Mid of June every year. Whenever, wilting of beet roots carried out at high temperature and low humidity for any cause, which are prevailing during the period from the end of April to Mid of June. So, this work was carried out at laboratories of Delta Sugar Company, Kafr El-Sheikh Governorate, Egypt, as well as Food Science and Technology Department, Faculty of Agriculture, New Valley Branch, Assuit University during 2016 and 2017 working seasons for eight days and replicated four times during the period from 25th April to 6th June.to identify the influencing of wilting degree (the loss% in moisture content of beet roots) postharvest on physiochemical traits, impurities contents and processing efficiency parameters of sugar beet roots.
The obtained results revealed that wilting degree of beet root had a significant effect on physical properties of sugar beet juice expressed as total soluble solids %(TSS%), pH value, bulk density (kg/m3) and color of raw juice (Icumsa units); impurities contents of sugar beet,i.e. ɑ-N, K and Na(millieq./100g), and chemical composition of sugar beet roots ,i.e. pol % , reducing sugars% and dextran content as well as processing efficiency parameters of sugar beet roots, i.e. juice purity%, sucrose recovery %, sugar losses% in waste, quality index of beet roots and weight losses % of beet roots.
We hope that the above-mentioned results in this work would help understand the changes which take place in sugar beet roots postharvest caused by the wilting, which cause significant economic losses in sugar production and to know the practices that reduce sugar loss during processing. Here, we demonstrate that all sugar beet growers and the processors’ benefit directly when postharvest losses are minimized. The increase in wilting degree of beet roots means make them lose their refreshment and affect negatively sugar extraction during manufacturing in sugar factories.
https://assjm.journals.ekb.eg/article_214395_3b0a09e8a0dbcafa2a49dddf92569fbe.pdf
Sugar beet
Pol %
wilting
Sr Genes
QUALITY INDEX
TSS% and ɑ-N
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
355
360
10.21608/assjm.2018.214396
214396
Original Article
Effect of storage period atcold storage on some physical and chemical properties of potato tuber prepared for processing
The aim of this study was to evaluate the effect of storage period and condition on the physical and chemical properties of potato tuber prepared for processing. Two varieties of potatoes namely Diamant and Santana were evaluated for their suitability for industrial production of chips and French fries with respect to storage period. The quality characteristics of the potato samples were examined during cold storage at 10 0C and 75-85% relative humidity and treated with (ChlorphenylIso- Propyl- N-3-Carbamate CIPC) as sprout inhibitor. Physical properties of potatoes were evaluated every 2 months for 6 months throughout 2 seasons. The results obtained showed that there was relative increase on dry matter content ranged between 21.70 % at zero time to 23.73 % at six months for Diamant variety and 21.53 % at zero time to 25.80 % at six months for Santana variety at both seasons. Also there was observed increase in specific gravity from 1.092 at zero time to 1.097 at six months for Diamant variety and 1.086 at zero time to 1.107 at six months for Santana variety at both seasons. For chemical parameters tested there was a decline in starch content 19.51% at zero time to 15.45 % at six months and 19.55 % at zero time to 16.70 % at six months for Diamant and Santana varieties respectively.. For chemical parameters tested there was a decline in starch content 19.51% at zero time to 15.45 % at six months and 19.55 % at zero time to 16.70 % at six months for Diamant and Santana varieties respectively. Accordingly there was an increase in reducing sugars from 0.234 % at zero time to 0.278 % at six months and from 0.233 % at zero time to 0.266% at six months for Diamant and Santana varieties, respectively, which affected both quality and consumer acceptability due tonon-enzymatic browning result in darkening of chips and French fries colour. The main recommendations of this study are that the storage condition must be controlled during storage period due to its critical effect on the quality characteristics of potato frying products.
https://assjm.journals.ekb.eg/article_214396_a5cb696e947266e8cc9584d023af69ab.pdf
Cold storage
potato
reducing sugars
Starch
Storage period
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
361
378
10.21608/assjm.2018.214399
214399
Original Article
Identification and determination of aroma components of some juice and its blends
ashraf sharoba
ashraf_sharoba@yahoo.com
1
Department of Food Technology, Faculty of Agriculture, Benha University, Egypt.
In this study, the chromatographic analysis method was used to determine the components responsible for the flavor in some types of juices and beverages in Egypt. The components of the flavor were also estimated in mango juice, orange juice, apricot juice, carrot juice and kaki juice after the addition of pectinase and cellulose enzymes with a concentration of 0.1% to determine the effect of these enzymes on the flavor components of this juice. And also prepared 4 mixtures including mango juice with orange juice, mango juice with carrot juice, mango juice with apricot juice and mango juice with kaki juice mixing rate 80:20% respectively. Flavor components were estimated in 11 samples. The results showed that the product of mango juice contained propane, 2-methoxy-content of 21.86 and apricot juice on 9-Octadecenoic acid (Z)with 14.59 and orange juice on compound ethane, 1,1'-oxybis-with 79.36 and kaki juice on ethane, 1,1'-oxybis compound with 84.39 and carrot juice on 9-octadecenoic acid (Z)-compound by 40.31. The addition of pectinase and celluloseal enzymes to mango juice increased 9-Octadecenoic Acid (Z)- by 86.88 and 96.29 respectively. And contain mango juice mixed with orange juice on 9-Octadecenoic acid (Z) compound with 54.91 and mango juice mixture juice carrots on the ethane, 1,1'-oxybis compound 70.35 and mango juice mixed with kaki juice ethane, 1,1'-oxybis on the compound 89.07 and mango juice mixed with apricot juice ethane, 1,1'-oxybis- on the compound 89.07.
https://assjm.journals.ekb.eg/article_214399_7c801a8ffeebf0a1891807b7d07ad9f5.pdf
Aroma chemical composition
aroma components, Mango juice, Orange juice, Apricot juice, Carrots juice, Juice blends
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
379
380
10.21608/assjm.2018.214401
214401
Original Article
Chemical and rheological properties of some Egyptian and Yemeni wheat varities (Abstract)
ashraf sharoba
ashraf_sharoba@yahoo.com
1
Department of Food Technology, Faculty of Agriculture, Benha University, Egypt.
In this study, the chromatographic analysis method was used to determine the components responsible for the flavor in some types of juices and beverages in Egypt. The components of the flavor were also estimated in mango juice, orange juice, apricot juice, carrot juice and kaki juice after the addition of pectinase and cellulose enzymes with a concentration of 0.1% to determine the effect of these enzymes on the flavor components of this juice. And also prepared 4 mixtures including mango juice with orange juice, mango juice with carrot juice, mango juice with apricot juice and mango juice with kaki juice mixing rate 80:20% respectively. Flavor components were estimated in 11 samples. The results showed that the product of mango juice contained propane, 2-methoxy-content of 21.86 and apricot juice on 9-Octadecenoic acid (Z)with 14.59 and orange juice on compound ethane, 1,1'-oxybis-with 79.36 and kaki juice on ethane, 1,1'-oxybis compound with 84.39 and carrot juice on 9-octadecenoic acid (Z)-compound by 40.31. The addition of pectinase and celluloseal enzymes to mango juice increased 9-Octadecenoic Acid (Z)- by 86.88 and 96.29 respectively. And contain mango juice mixed with orange juice on 9-Octadecenoic acid (Z) compound with 54.91 and mango juice mixture juice carrots on the ethane, 1,1'-oxybis compound 70.35 and mango juice mixed with kaki juice ethane, 1,1'-oxybis on the compound 89.07 and mango juice mixed with apricot juice ethane, 1,1'-oxybis- on the compound 89.07.
https://assjm.journals.ekb.eg/article_214401_1c027ce3936589b82a3cecba5122139e.pdf
Aroma chemical composition
aroma components, Mango juice, Orange juice, Apricot juice, Carrots juice, Juice blends
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
381
382
10.21608/assjm.2018.214645
214645
Original Article
Improvement of glycine oxidase by DNA shuffling, and site-saturation mutagenesis of F247 residue (Abstract)
Glyphosate is a broad spectrum herbicide widely used throughout the world, and it could be degraded by glycine oxidase (GO) through cleavage of C-N bond. For a better understanding of the structure-function relationship and improving activity of B3S1 (GO from Bacillus cereus) (Zhan et al, 2013), DNA shuffling was performed. A mutant B4S7 (Km, Vmax, kcat and kcat/Km on glyphosate were 0.1 mM, 0.002401 mM min-1,3.62 min-1 and 36.2 mM-1 min-1, respectively. The four parameters on glycine were 50.34 mM, 0.02098 mM min-1, 2.18 min-1 and 0.04 mM-1 min-1, respectively) was obtained from 10,000 clones, which presented a 3.9-fold increase of specificity constant (the kcat/Km ratio between glyphosate and glycine) compared with B3S1. Especially, the Km value of B4S7 to glyphosate was much less than those reported GO. Structure modeling and molecular docking indicated that the novel mutation point F247S was close to the active site of the enzyme. To identify the role of the site, the remaining 19 amino acids were introduced into the site by site-saturation mutagenesis. The result showed that compared with B3S1, the specificity constant of mutant F247S and F247R increased 0.64-fold and 1.04-fold, separately. While to F247E, it decreased 2.01-fold. Therefore, the site 247 plays a crucial role in regulating substrate specificity. This study provides new information on the structure-function relationship of glycine oxidase and the development of glyphosate tolerance crops.
https://assjm.journals.ekb.eg/article_214645_e3aadcc00ab6915388c1084433e21c1b.pdf
Glycine oxidase by
DNA shuffling
site-saturation mutagenesis
F247 residue
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
383
400
10.21608/assjm.2018.214647
214647
Original Article
Cytological and Molecular studies in some interspecific hybrids resulted from crossing between tetraploid and hexaploid wheat
Makhlouf Bekhit
makhlouf.bakhit@fagr.bu.edu.eg
1
Faculty of Agriculture at Moshtohor, Benha University
Wheat relatives are rich sources of beneficial genes that are capable to change the situation of the wheat strains. The present study attempted to cross three tetraploid wheat strains as male parents with five strains of hexaploid bread wheat to understand the behavior of hybrids in relation to cytology, Scanning Electron Microscopy for wheat grains and SDS-PAGE (Friabilin). Scanning electron microscopy of dry grains revealed morphological details of grain surface structure. The grains of strain 20 cultivar showed narrower size and hairless area than the grains of strain 9 or 17 or 6. The hairy end of the strain 17 grains is broader than the same end (hairy) of the strain 21. The hairy end of the strain 24 is completely disappeared from grains. All the produced six crosses were containing hairy ends as an indicator for the inheritance of this character. Probably complete dominance of the gene controlling the brush area in the wheat grain. Various degrees of chromosome anomalies were seen with all the hybrids between T. durum and T. aestivum. D genome from T. aestivum could enhance more lagging chromosomes rather than genomes A and B of cultivated species. The main observed abnormalities in the produced crosses are the laggard formation. Fraibilin clearly associated with genetic proximity of the species, which characterized themselves by maintaining the genome homeology.
https://assjm.journals.ekb.eg/article_214647_aa88b4636119dfc9518f4351854a61f2.pdf
wide hybridization
Triticum
SDS-PAGE
Fraibilin
Laggards
meiotic anomalies
tetraploid wheat
Bread wheat
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
401
410
10.21608/assjm.2018.214649
214649
Original Article
Identified SSR Marker Linked to Leaf, Stripe and Steam Rust Resistance Genes in Some High Yield Potential Bread and Durum Wheat varieties
Hoda El-Gharbawy
hodaelgharbawy@yahoo.com
1
Wheat Research Department, Field Crops Research Institute, Agricultural Research Center, Giza station.
This study was conducted to identify SSR markers linked to leaf, stripe and stem rusts resistance genes Lr34, Lr 19 and Lr 47, Yr 10 and Yr 15 and Sr 2, Sr12, Sr 24, Sr 26#43 and Sr 25 to be used as markers for identifying the rust resistance genes in seventeen Egyptian bread and durum wheat varieties and two promising lines produced from doubled haploid plants. Bread wheat varieties (Misr 1, Giza 168, Gemmieza 10, Nubaria 1, Sahel 1, Sids 13, Sids 4, Sakha 94, Gemmeiza 11, Giza 171, Gemmeiza 9, Sids 1 and two doubled haploid promising lines DH 2 and DH3) and durum wheat varieties (Bani Suef 5, Bani Suef 6 and Sohag 3). The materials were planted in the two successive growing seasons 2015/2016 and 2016/2017 at El-Giza research station to determine some characters, i. e., No. of kernels/spike, No. of spikes/m2, 1000-kernel weight(g) and grain yield Ardab/faddan. Moreover, materials were tested to rust diseases in Sakha and Nubaria stations in 2015/2016 and 2016/2017. Significant differences were found among varieties in the two growing seasons, in all characters in this study. DH#3 promising line recorded the highest value in No. of spikes/m2 and grain yield ardab/faddan in the two growing seasons. Misr 1, Giza 171, DH#2 and DH#3 had no infection of rust reaction in the two growing seasons for the three types of rusts, but Sids 13 and Nubaria 1 were 5MS (moderate susceptible) for Yr rust and 10 MS for Lr rust. However, Sr rust reaction zero no infection in all genotypes. Yr 10 and Yr 15 stripe rust resistant gene used in this study, positive molecular marker Linked to Yr 10 detected in Misr1, Sids 1and DH3, while Yr 15 was detected in Misr 1, Giza 168, Sahel 1, Sids 4, Sids 1, Bani Suef 5, Bani Seif 6, DH#2 and DH#3. Lr 34 leaf rust gene was found in Sids 13, Sakha 94 and DH#3, while Lr 47 gene was detected in Misr 1, DH#2 and DH#3, but Lr 19 was detected in any genotype. Sr 2 durable resistant stem rust gene was detected in all genotypes expect Sids 1, moreover Sr 26#43 detected in all genotypes too expect Sids 4 variety. Sr 12 gene was detected in Misr 1, Giza 171, Gemmeiza 9 and DH# 3. All genotypes had no Sr 24, While Sr 25 resistance gene was detected in Misr 1, Giza 171 and DH#3 genotypes. Dedication of more than one gene in any variety is useful for durable resistance. Seven rust genes were detected in Misr 1 for the three rusts and eight genes were found in promising line DH#3, which caused resistant to rust. So, Misr 1 and DH#3 are considered as promising bread wheat high yielding genotypes and resistant to rust diseases can which be used in breeding program, as pre breeding in hybridization program.
https://assjm.journals.ekb.eg/article_214649_dabef45ce7546903fbcc0122d58f4bbc.pdf
bread
durum
Wheat
Resistance gene
SSR marker
yield
rust diseases
rust reaction
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
411
414
10.21608/assjm.2018.214651
214651
Original Article
Homology Modeling, Structure and Active Site Prediction of Stem Rust Resistant Protein in Wheat
Homology modeling is very helpful tool which produces 3D models when the target and template are closely related. The stem rust resistance gene Rpg1 encodes RPG1 protein, which plays a complex role in stem rust resistance. Homology modeling of RPG1 is important to study its role and pathway. The homology modeling of this protein was done using MODELLER 9.11. Then models of same proteins were superimposed to check the validation. NIH server containing tools, ERRAT was used to evaluate the 3D structure of protein RPG1, ERRAT score for this model was 77%. This score was further improved by doing loop optimization of RPG1 using similar tool. It was improved upto 80%. 3D model of stem rust resistance was further used to analyze the activity of protein. Active sites of RPG1 were detected by using ADDS (Automated active sites detection, docking and scoring). Total of 15 active sites were identified and active sites with highest score were used in further studies. The information achieved may be useful to understand the biology of stem rust disease in wheat which may further help to manipulate the disease mechanism.
https://assjm.journals.ekb.eg/article_214651_5726bdc80e3f2ffaa1521683a393db89.pdf
stem rust
Triticum aestivum
Homology Modeling
RPG1 protein
active site prediction
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
415
422
10.21608/assjm.2018.214653
214653
Original Article
Molecular and morphological screening for net blotch resistance in some Egyptian barley cultivars
Ismael Khatab
ismael.khatab@yahoo.com
1
Department of Genetics faculty of Agriculture Kafrelsheikh University Egypt
Molecular and morphological screening analysis was evaluated to study the similarity levels and marker assisted selection associated with resistance to net blotch. Sequence-Related Amplified Polymorphism (SRAP) as a new marker was used on Egyptian barley for net blotch among 10 Egyptian barley cultivars. Five traits during two consecutive seasons and identify their reaction net blotch. The results exhibited a significant difference among studied cultivars for all traits. The highest mean values for all studied traits detected for, Giza 2000 and Giza 132 which they show resistance to net blotch. Molecular marker analysis using six selected primer combinations gave 56 total bands. Primer combination me5+em5 gave the highest polymorphism (100 %) and the highest polymorphic information content PIC was (0.96). However, primer combination me5+em4 generated specific band associated to net blotch with size ~1090 bp. The dendrogram clustered all studied cultivars into two main clusters each includes the most closed cultivars together according their reactions to net blotch. Results showed that the SRAP marker could be efficiently used to assess genetic variation among Egyptian barley and will be useful for barley germplasm management in terms of biodiversity protection and design of new crosses for disease resistance to net blotch breeding program.
https://assjm.journals.ekb.eg/article_214653_5b13b09d3d77b26a69332953a25aa560.pdf
Hordeum vulgare
net blotch (Pyrenophora teres) Sequence-related amplified polymorphism (SRAP)
UPGMA cluster analysis
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
423
436
10.21608/assjm.2018.214655
214655
Original Article
Physiological and molecular genetic studies for cotton leaf worm (Spodoptera littoralis) tolerance on six Egyptian soybean cultivars
Marwa M. Ghonaim
marwa_ghonaim2006@yahoo.com
1
Field Crops Research Inst., Agricultural Research Center, Giza, Egypt
Cotton leaf worm (Spodoptera littoralis) is considered one of the most destructive agricultural pests. Six soybean cultivars (Giza-21, Giza-22, Giza-35, Giza-82, Giza-83 and Giza-111) were grown under natural infection with cotton leaf worm. The effect of two elicitors, methyl jasmonate and sodium nitroprusside on enhancing the ability of susceptible cultivars to tolerate (Spodoptera littoralis) was studied. Giza-35 and Giza-111 showed tolerance performance under natural infection compared to Giza 22 and Giza 82 as sensitive ones, while Giza 83 and 21 showed moderate tolerance. Both treatments positively affected seed yield and its components and fatty acid composition. Extracted fatty acids showed great changes in treated plants comparing with the untreated controls. Plants treated with the two elicitors showed an increase in Linoleic acid and Linolenic acid fatty acids and decrease in Palmitic acid and Palmitolic acid content. Treatment with methyl jasmonate was found to be more effective than sodium nitroprusside and enhanced resistance of the susceptible cultivars. Eight IRAP and iPBS retrotransposon-based markers were used to detect genetic differences among studied soybean cultivars and to develop molecular genetic markers for cotton leaf worm infestation. The technique successfully identified soybean genotypes in addition to nineteen molecular markers related to soybean tolerance.
https://assjm.journals.ekb.eg/article_214655_b57fcad057e54c3b672c54dadff8350d.pdf
Soybean
Methyl jasmonate
Sodium nitroprusside
fatty acids
IRAP-iPBS
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
437
448
10.21608/assjm.2018.214657
214657
Original Article
Bioinformatics and differential expression analysis of chalcone synthase genes (CHS1, 2, 3) under gamma rays elicitation in Silybum marianum L.
Hoda El-Garhy
hoda.algarhy@fagr.bu.edu.eg
1
Moshtohor, Tukh
Silymarin is a constitutive secondary metabolite extracted fromSilybum marianumseeds with wide therapeutic and medical effects. Gamma irradiation could be a powerful tool for obtaining promising mutated genotypes of S. marianum with high contents of flavonolignans. The transcript levels of the chalcone synthase genes (CHS1, CHS2, and CHS3), encoded for silymarin, in response to two different gamma irradiation doses (200 and 600GY) were determined using qRT-PCR. Also, Molecular isolation and characterization of chalcone synthase genes and its encoded protein were investigated via bioinformatics tools. Results of qRT-PCR confirmed that exposure of S. marianum seeds to gamma irradiation up-regulated the expression of the CHS1, CHS2 and CHS3 genes which could be positively correlated with increasing of silymarin content in the fruits. In conclusion, the increasing of flavonolignans content conflicts a great contribution in the field of medicine.
https://assjm.journals.ekb.eg/article_214657_3e2b6bfa143652b2ecc69068c3d6519a.pdf
S. marianum
gamma radiation
qRT-PCR
Sequencing
Chalcone synthase genes
Bioinformatics
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
449
456
10.21608/assjm.2018.214658
214658
Original Article
Morpho-molecular genetic assessment of five Quinoa (Chenopodium quinoa Willd) genotypes cultured under Egyptian climate conditions
Four introduced quinoa genotypes, moreover one recently domesticated variety were utilized as a part of the present study. Domesticated variety acquired the name Giza1, while the other introduced genotypes named Q18, Q21, Q22, and Q29. First genotype obtained from Denmark, the other genotypes introduced from Chile. All genotypes cultured in a randomized complete block design with four replications in two locations Moshtohor in Qalyubiya governorate, and Nabtit at Sharqia governorate. This investigation included six imperative attributes influencing the yield, 1) plant tallness (cm), 2) number of branches/plant, 3) primary head weight/plant (g), 4) weight of the 1000-grain (g), 5) grain yield weight /plant (g), 6) yield weight (kg/ha), 7) life cycle length (day).Statistical analysis of the two characters branches number / plant, and life cycle length detects no significant differences between all genotypes. At the other side plant height with Q29 showing the lowest height 111.63 cm. Genotype Q18 was significantly the tallest (119.75).Main head weight gave lowest reading with the variety Giza1 (16 gram). The greatest value obtained from the genotype Q22.Fourth trait was grain yield per plant. Lowest means were obtained from the two genotypes Giza1 and Q18 with 13.12 and 19.75 gram respectively, while the two genotypes Q22, and Q29 record best results with means 19.38, and 19.5 consequently. Regarding 1000-grain weight the variety Giza1 gave also weakest reading 3.21 gram. At the other hand genotype Q22 gave superior 1000-grain weight 3.49 gram. Grain yield/ ha show weakest results with Q21, Giza1, and Q22 with means 2.14, 2.16, and 2.39 ton / ha respectively. Genotype Q29 gave 3.32 ton grain yield / ha. Total protein extracted from the five genotypes, to study the optimum conditions for preparing the protein isolate of quinoa seeds and investigates the physicochemical and functional properties of the isolated protein to assess the potential use of quinoa protein isolate in food applications and manufacturing. The protein isolate of quinoa was obtained by protein solubility at alkaline pH value, followed by precipitation at an acidic pH values (2, 4, and 6). SDS–PAGE showed protein bands with 177,8KDa and 34,91KDa potentially corresponding to cheno protein in some extraction pHs. Quinoa protein had reasonable concentrations of essential amino acids (except tryptophan) with a high level of lysine (17.13%). A sharp maximum solubility was observed at the pH value (4.0), and the maximum value was observed at the alkaline. Quinoa protein showed a high In Vitro digestibility.
https://assjm.journals.ekb.eg/article_214658_f4225b491ea290666394e01b1cce19e3.pdf
Chenopodium quinoa
genetic assessment
SDS-PAGE
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
459
472
10.21608/assjm.2018.214661
214661
Original Article
Morphological and genetic variations in "Balady" Mandarin induced by gamma irradiation
Induced mutagenesis using gamma ray has been proven applicable to improve varieties of many genotypes of crop species. The present investigation was carried out to study genetic variability induced by gamma rays in Balady mandarin. Bud woods were irradiated with doses of 0, 2, 4, 6 and 8 kr along with control and budded onto volkamer lemon rootstock. Bud survival percentage decreased with increasing dose of gamma radiation. Doses of 6 and 8 Kr were lethal where the LD50 was achieved at 2.1 Kr. Shoot length, leaf number per plant, leaf area per plant and stomata width decreased significantly by increasing the gamma dose from 0 to 4 Kr. Whereas, stomata number per unit area increased significantly compared to control. Molecular analysis of some induced mutants using ISSR and RAPD markers revealed that, the polymorphism percentage ranged from 50% to 80% when the ISSR primers were used and from73.33% to 100% for RAPD primers. The 4 Kr treatment caused the highest polymorphism percentage (69.05% and 85.32%), whereas the 2 Kr treatment had 62.5% and 74.23% for the ISSR and RAPD primers respectively. Gamma irradiation treatments included alteration in protein bands intensity, appearance of new bands and disappearance of other bands. The most visible changes in protein patterns were the appearance of new bands with molecular sizes of 264, 196 and 139 KDa in some selections of 4 Kr treatment, and bands of 87, 86, 82, 51, 45, 30 and 9 KDa in selections of 2 and 4 Kr treatments. The 4 Kr treatment achieved more genetic variation than the 2 Kr treatment.
https://assjm.journals.ekb.eg/article_214661_7741286963c2291fb8593fa71a2cc424.pdf
mandarin
Gamma rays
Genetic variability
molecular markers
Protein pattern
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
475
486
10.21608/assjm.2018.214664
214664
Original Article
High-level yielding of insecticidal crystal proteins and field testing of a genetically modified Bacillus thuringiensis biopesticide WG-001
Lin Li
lilin@mail.hzau.edu.cn
1
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
A genetically modified (GM) Bacillus thuringiensis strain WG-001 was constructed and prepared as a biopesticide for field trial of its biocontrol efficacy and for assessing the ecological impact on the indigenous microflora. Using a TnpI-mediated site-specific recombinant strategy, a cry1Ac10 and an additional p20 gene were transferred into B. thuringiensis wild-type strain YBT-1520 while all of the heterologous antibiotic resistance genes were selectively eliminated. The resultant engineered strain, WG-001, exhibited a two-fold increase of the total Cry proteins and an approximately 25% increase of lepidopterancidal potency in fermentation compared to its parent strain, and it was also given a distinct trait to allow tracking of its appearance using simple polymerase chain reaction verification of p20 and cry1-like genes. In the cotton field, released WG-001 biopesticide showed a limited aerial dissemination capability and dispersal range, and did not establish a population in the soils or on the cotton leaves of the field. There was no evidence supporting the significant biological influence of WG-001 on the indigenous microflora. These results demonstrate that the likelihood of risks of released WG-001 to non-target organisms in the cotton fields was limited by the low survival of the introduced strain.
https://assjm.journals.ekb.eg/article_214664_8ac8ed5c47bb46c8d594b9268903a009.pdf
Genetically modified Bacillus thuringiensis
pesticide
Fate
Cotton field
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
487
492
10.21608/assjm.2018.214668
214668
Original Article
Laboratory tests on the integrated management of cotton leaf worm Spodoptera littoralis (Boisd.) by using some insecticides and entomopathogenic nematodes
Hassan Hassan
dr_hassan_m_hassan2000@yahoo.com
1
Department of Plant Protection, Faculty of Agriculture , Minia University, Minia, Egypt
Effects of certain insecticides i.e. Coragen, Nomolt, Ekio and Magic smart with two species of entomopathogenic nematodes, Steinernama carpocapsae and Heterorhabdits bacteriophora were tested against cotton leaf worm Spodoptera littoralis. All tested insecticides caused less mortality to the tested entomopathogenic nematodes. Magic Smart significantly surpassed other insecticides causing the mortality of 11.6 and 13.8 % to the entomopathogenic nematodes S. carpocapsae and H. bacteriophora opposite to Coragen that caused slight mortality, 2.5 and 4.4%, respectively and didn’t differ significantly than the check. Nomolt and Ekio gave mortality of 5.4 and 8.8 % to S. carpocapsae as well as 8.8 and 12.0 % to H. bacteriophora respectively. These results show that the used insecticides have less effect on entomopathogenic nematodes specially S. carpocapsae. The entomopathogenic nematode S. carpocapsae exposed to Coragen, Nomolt, Ekio and Magic smart gave 92.5, 85.0, 80.0 and75.0 % mortality to cotton leaf worms 5th larval instar, respectively. The entomopathogenic nematode H. bacteriophora exposed to Coragen ,Nomolt, Ekio and Magic smart gave 83.4, 77.2, 71.0 and 60.6 % mortality % of S. littoralis, respectively. Joint effect of insecticides and entomopathogenic nematodes show that the mixture between S. carpocapsae with each of Coragen, Nomolt, Ekio and Magic smart at the quarter lethal concentration (LC25s) per each gave synergism, synergism, additive and additive effects, respectively. As while H. bacteriophora mixed at the LC25 with the LC25s of Coragen, Nomolt, Ekio and Magic Smart attained synergism, additive, additive and additive effects, respectively.
https://assjm.journals.ekb.eg/article_214668_4718f6a8eec4097de4af23c78c87f4fa.pdf
Entomopathogenic nematodes
Co-toxicity
S.carpocapsae
H. bacteriophora
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
493
496
10.21608/assjm.2018.214671
214671
Original Article
Effect of several eco-friendly materials on total phenols of two squash varieties
Mohamed Azab
mohamed.azab@fagr.bu.edu.eg
1
Plant Protection Department, Faculty of Agric., BenhaUniv.,Qalubia, Egypt
To study the effect of several environment-friendly materials on total phenols of two varieties of squash, field experiments were carried out on squash (Cucurbitapepo L.) cv. Maharaja and Eskandarani during 2015, early summer, summer and nili cultivations. The results indicated that in cultivar Maharaja, the different treatments reduced the total phenol contents of leaves as following order, a mixture oil of camphor + ginger (78.19%) > ginger oil (58.78%) > methomyl (54.02%) > a mixture of camphor oil + lequrice root extract (50.12%) > spinetoram (45.16%) > a mixture of ginger oil + lequrice root extract (35.49%) > camphor oil (33.91%) > lequrice root extract (31.17%) > azadirachtin (29.15%) > spinosad (25.63%), whereas in Eskandarani cultivar this reduction order was ginger oil (55.36%) > methomyl (55.25%) > azadirachtin (51.30%) > a mixture oil of camphor + ginger (40.40%) > a mixture of ginger oil + lequrice root extract (37.07%) > camphor oil (34.69%) > a mixture of camphor oil + lequrice root extract (29.96%) > spinosad (29.24%) > spinetoram (27.77%) > lequrice root extract (17.83%). Moreover, it was observed fluctuations in total phenol contents of squash leaves among its three cultivations, early summer, summer and nili when treated with varied eco-friendly materials as well as between the two cultivars studied. It was concluded that the environment-friendly materials have a negative effect on total phenol contents of squash leaves and the interaction among cultivars of squash, season of cultivations and environment-friendly materials plays a great role on this effect.
https://assjm.journals.ekb.eg/article_214671_d41d8cd98f00b204e9800998ecf8427e.pdf
Environment-friendly materials
Cucurbita pepo
Maharaja
Eskandarani
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
497
508
10.21608/assjm.2018.214673
214673
Original Article
Biological control of tomato wilt disease incited by Fusarium oxysporum f.sp lycopersici and there effects on seed germination and other biological parameters
O. I. Saleh
salehelkhashab@gmail.com
1
*Plant Pathology Dept., Fac. Agric. El-Minia Unv. Egypt.
In previous paper it has been reported that Fusarium oxysporum f.sp < /strong> lycopersici was the incident of Fusarium wilt disease in some Egyptian governorates (Saleh et al., 2016).This paper delt with biological control of the pathogenic fungus. Trichoderma harzianum , Trichoderma viride, Bacillus subtilis and Bacillus megaterium were isolated from rhizospher of naturally wilted infect plant and identified as abovementioned . The bioagents showed strong antagonistic effect in vitro and reduced disease severity under filed conditions. T. harzianum proved more powerful than T. viride in reducing disease severity while B. subtilis proved slightly more powerful than B. megaterium under this investigation. Moreover, T. harazianum, B. subtilis and Rhizo – N were more powerful indecreasing disease severity than other tested bioagents and surpassed the plant – guard and Moncren fungicide. Culture filtrate of Fusarium oxysporum f. sp Lycopersici reduced seed germination of tomato and affected other botanical parameters indicating that it is hazardous and notorious.
https://assjm.journals.ekb.eg/article_214673_87b441bb5c0f82961435252923cd4ef0.pdf
Fusarium oxysporum f.sp Lycopersici
Trichoderma spp
Bacillus ssp
Rhizo – N
Plant guard
Monceren Compy
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
509
518
10.21608/assjm.2018.214674
214674
Original Article
Rearing of the red palm weevil, Rhynchophorus ferrugineus (Olivier) on different natural diets.
I.R.M El-Zoghby
ielzoghby77@agr.aswu.edu.eg
1
Plant Prot. Dept., Faculty of Agric. and Natural Resources, Aswan Univ., Aswan, Egypt
Five different diets (Small pieces of sugarcane stems, Sugarcane stem pieces + Sugarcane residues, Sugarcane stem pieces + Date palm trunk pieces, Sugarcane stem pieces + Grinded frond of date palm and Sugarcane stem pieces + Food residues of RPW) were tested for rearing the red palm weevil Rhynchophorus ferrugineus and to investigate the effect of diets on some biological aspects under laboratory conditions. Larval durations of different instars were affected by different diets.
The larvae fed on (Sugarcane stem pieces + Grinded frond of date palm), slowly developed to exhibit the longest duration of 103.78 days. Meanwhile, the larvae fed on the other diets showed a gradual shortage in larval periods to give means of 89.47 and 74.63 days when fed on Small pieces of sugarcane stems and Sugarcane stem pieces + Food residues of RPW, successively.
Feeding on different diets had great effect on the means of the pupal period, which were obtained between the tested diets. Longevity of males and females emerged from larvae fed on the different tested diets varied.
The sex-ratio of emerged adults was the highest (more females), when larvae were fed on sugarcane stem pieces + food residues of RPW. Mean number of eggs deposited by emerged females from larvae fed on different diets was highest in case of (Sugarcane stem pieces + Food residues of RPW). The mean of incubation period of eggs laid from adult fed on three tested diets ranged from 3.78 to 4.54 (Sugarcane stem pieces + Food residues of RPW and Sugarcane stem pieces + Grinded frond of date palm, respectively. Hatchability percentage proved to be affected by different diets. The superior diet were sugarcane stem pieces + Food residues of RPW, because of biology completed and the diet kept moisture and remained fresh all the time.
https://assjm.journals.ekb.eg/article_214674_ed295415fb6fe24826654c4efd160c3f.pdf
biological aspects
feeding diets
Rhynchophorus ferrugineus
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
519
530
10.21608/assjm.2018.214678
214678
Original Article
Test of the effectiveness of the herbicides Lumax and Camix with the presense of wheat crop Triticum aestivum L. residues in the control of yellow maiz Zea mays L. 5018
Ammar A. Obaid
mmaar79@yahoo.com
1
Directorate of Agriculture Salahuddin, Iraq
The experiment was conducted in one of Salah Aldeen province’s fields in Aldjel city during the fall season of 2016.This experiment used herbicides namely Lumax with the active material of S-metochlor+terbthylazine+Mesotrion at the recommended 3L.h dose in presence and absence of the residue from the last season and half of the recommended dose in presence and absence of the residue, all have done with and without herbicide control. A second herbicide namely Camix with an active ingredient of S-metochlor +Mesotrion was used at the recommended dose in presence and absence of the residue from the last season and half of the recommended dose in presence and absence of the residue, all have done with and without herbicide control. The experiment was implemented according to Randomized Complete Block Design (RCBD) with three repetitions. The results showed that percent of control increases for all blocks in wide and slim leaf at different concentrations and types of herbicides. Camix showed highest percentage of control at 2.8 L/h for slim leaf in absence of residue, Lumax showed highest percentage of control at 1.5L/h for wide leaf in present of residue and 3 L/h in absence of residue. Treatment in present of residues showed the highest length of plant, 202 cm, at 3 L/h of Lumax for the slim leaf and the highest leaf area and leaf area index(LAI) , 4890 cm2 and 26.1 respectively. The highest total yield obtained was 3801 ton/h at 3 L/h of Lumax absence of residues.
https://assjm.journals.ekb.eg/article_214678_fc55ee2b99c5aa0f42ef29622b1ef440.pdf
yellow corn
weed control
Camix
Lumax
Leaf area
LAI
Corn yield
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
531
532
10.21608/assjm.2018.214699
214699
Original Article
Deciphering the effects of long-term fertilization practices on the Nitrite-oxidizing bacterial community in a Black soil (Abstract)
Wenli Chen
wlchen@mail.hzau.edu.cn
1
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China
Nitrite-oxidizing bacteria (NOB) have been recognized as important members for nitrogen cycling in agricultural ecosystems, and how NOB respond to long-term fertilization and variations at soil aggregate levels remain unclear. In this study, the potential nitrite oxidation activity(PNO) and the abundance of nitrite oxidizers were examined in three aggregate fractions (2000-250, 250-53, <53 μm) of a Black soil under four fertilization regimes. Pyrosequencing was utilized to characterize the composition and distribution of Nitrobacter- and Nitrospira-like NOB populations. PNO was higher in microaggregates (250-53μm) and lowest in silt+clay fraction (<53μm). Fertilization affected PNO to a higher extent than the aggregate sizes. The maximum abundances of Nitrobacter and Nitrospira were observed in macroaggregates (2000-250μm), followed by microaggregates and silt+clay. Dominant (relative abundance >1%) Nitrobacter OTUs were phylogenetically related to some known groups of Nitrobacter environmental clones and those of Nitrospira were affiliated with the Namibia soil cluster, Nitrospira lineage II and V, and Unknown affiliation. Multi-response permutation procedures analysis indicated that nitrifier community was strongly affected by the fertilized regimes but that the aggregate effects were only detectable in the unfertilized soils. Redundancy analysis showed that the community composition of Nitrobacter- and Nitrospira-like NOB was affected by soil total phosphorus, available phosphorus, total potassium and NH4+ content. Our findings suggest that fertilization treatments rather than soil aggregates play more important roles in shaping NOB community structure.
https://assjm.journals.ekb.eg/article_214699_e956c302b5ff30a27ab2bf553d426c86.pdf
Nitrite-oxidizing bacteria
potential nitrite oxidation
black soil
longterm fertilization
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
533
542
10.21608/assjm.2018.214701
214701
Original Article
Maize(Zea mays) biofertilization with B.megaterium and chemo-fertilization with rock or soluble P: Available N,P,K,Fe,Mn,Zn and Cu residual in soil after harvest.
Ahmed S. Al-Ahmadi
alahmadi_2014@yahoo.com
1
Application of P-biofertilizer (Bacillus megaterium )and P-chemical fertilizers (rock phosphate and superphosphate) with and without organic farmyard manure was studied in a 3-factor field experiment on maize (Zea mays ). Factors being P-biofertilizer(B), P-chemical fertilizer(P) and organic manure(M). Treatments being: B0 (no-biofertilizer), and B1 (P-dissolving B.megaterium bacteria), P0 (no P), P1 (rock phosphate 70 kg Pha-1) and P2(calcium superphosphate 35 kg P ha-1), M0 (no manure), M1 (24 Mg ha-1) and M2 (48 Mg ha-1). Treatment not receiving any material gave 3.62 Mg grains ha-1 . Applying any or more of the materials increased grain yield by as low as 1% by B0P0M1 to 13% by B0P0M2 , up to 87% by B1P2M0 and as high as 129% by B1P2M2..Increase main effects were: B = 40.4% , P = 25.6% for P1 and 20.0 % for P2; although P2 surpassed P1 in presence of the biofertilizer which enhanced soluble P-fertilizer.M= 5.1% for M1 and 20.0% for M2 effictive in presence or in absence of the biofetilizer. N,P,K,Fe,Mn,Zn and Cu residual in soil after harvest increased were by up to , 38,84, 42, 112, 269, 78, and 83 % for each respectively ; and highest availble contents were 91, 9.8, 308, 4.94, 1.55,1.51 and 0.42 mgkg-1 for each respectively.
https://assjm.journals.ekb.eg/article_214701_afba54984666ef4c765fd234997a7988.pdf
biofertilizer
B.megaterium
Zea mays
rock P
organic manure
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
543
556
10.21608/assjm.2018.214702
214702
Original Article
Effects of nano, chelated and conventional iron as soil addition on growth, anatomy and early yield of cucumber plants (cucumus sativus l).
Heba Elabd
heba.alabd@fagr.bu.edu.eg
1
Mostafa Helmy
Two greenhouse experiments in Randomized complete block design with four replications were conducted at Soil, Water and Bioenergy Resources, South Centers, Piketon, OH, Ohio State University, USA, to study the effect of iron forms (conventional Ferric chloride (FeCl3- 6H2O),chelated iron(6% Fe) and Nano Iron Oxide (Fe2O3, alpha, 99%,)at different doses(0, 50 and 100 mg/kg-1 soil) on growth, anatomy and early yield in cucumber plants (Cucumus sativusL.) cv.(Tyria F1) during 2015 and 2016 seasons. Results showed that, the different applied treatments increased plant height, leaf number, leaf area (LA) (cm2), fresh weight and dry weight at60 and 90 days after transplanting during the both seasons of cultivation. Also, anatomical studies were carried out on stems i.e.(stem thickness, xylem and Phloem thickness, and numbers, cambium region thickness, Fiber tissue thickness and pith thickness) and leaves measurements i.e.(plaside and spongy tissue and xylem thickness, number of vessels and phloem at 30 days of plant age in the second season. Data showed positive response to the different applied treatments especially with nano iron at 100 mg/kg-1.In addition, early fruits number/plant, fruit length, fruit diameterand earlyfruit weight per plant increased due to the different applied treatments in the first and second seasons. Results also, showed that soil application of nano iron at 100 mg/kg-1 is the most effective treatment in this respect compared with other tested iron sources and control treatments.
https://assjm.journals.ekb.eg/article_214702_5c3d8761e9b6580ee99110085f5e76e1.pdf
nano ferric
chelated
Growth
Early yield
anatomy
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
557
572
10.21608/assjm.2018.214704
214704
Original Article
Impact of soil and foliar fertilization on yield and net income of maize plants alluvial clay soil.
esmat Noufal
esmat.noufal@yahoo.com
1
The study was aimed to investigate the effect of methods and levels of nitrogen, phosphorus and potassium application on maize growth, yield components, yields, nutrient uptake as well as net income. Two field experiments were carried out in the at Agricultural Farm of Sids Agricultural Research Station, ARC, Beni – Swif Governorate, Egypt during the two successive seasons of 2013 and 2014.
The nitrogen treatments were 120 kg N/fed as soil application (N1), 90 kg N/fed as soil application + two time foliar spraying of 2% urea solution (N2) and 60 kg N/fed as soil application + two time foliar spraying of 2% urea solution (N3). The phosphorus treatments were 13.6 kg P/fed as soil application (P1) , 6.8 kg P/fed as soil application + two time foliar spraying of 0.33% (P2), and two time foliar spraying of 0.33% P (P3). The potassium treatments were 39.8 kg K/ fed as soil application (K1) , 19.9 kg K/fed as soil application + two time foliar spraying of 2% potassium sulphate solution (K2) , and two time foliar spraying of 2% potassium sulphate solution (K3). A factorial experiment design included 27 treatments with four replications was performed. The results reveal that maize plant height, dry weight/plant, number of rows / ear , number of grains/ row , 100 – grain weight , grain , stover and biological yields as well as nutrient uptake and net income were significantly responded to methods and levels of N,P and K application. On the other hand combined soil application with foliar spraying (90 kg N/ fed with foliar spraying of 2% urea solution, 6.8 kg P/fed with foliar spraying of o.33% P or 19.9 kg K/fed with foliar spraying of 2% potassium sulphate solution) gave higher values , of growth parameter except plant height with 120 kg N/fed which recorded the tallest maize plant . In general, the recommended treatment for quality and quantity as well as the net income of maize was 90 kg N/ fed as soil application in combination with foliar spraying of 2% urea solution + 6.8 kg P/fed as soil application in combination with foliar spraying of 0.33% P + 19.9 kg K/fed as soil application in combination with foliar spraying of 2% potassium sulphate solution.
https://assjm.journals.ekb.eg/article_214704_0a7fd5f72dd1365a59d618a5ba8c07f9.pdf
NPK fertilization
soil application
foliar spraying
growth parameters
yield and its components
nutrient uptake and net income
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
573
590
10.21608/assjm.2018.214706
214706
Original Article
Effect of Rhizobium inoculation and foliar spray with salicylic and ascorbic acids on growth, yield and seed quality of pea plant (Pisum sativum L.) grown on a salt affected soil, New Valley-Egypt
esmat Noufal
esmat.noufal@yahoo.com
1
Department of Soils and Water, Faculty of Agriculture, Benha University, Egypt.
A field study was carried out to investigate the effects of seed inoculation with bio-fertilizer (Rhizobium), foliar spray with antioxidants i.e., salicylic acid (SA), ascorbic acid (AA) and SA+AA at 250 mg L-1 of each and their interactions on growth, yield components and some seed bioconstituents of pea plant (Pisum sativum L.) cv. Master-B grown on a salt affected sandy loam soil (EC=9.65 ds m-1), New Valley-Egypt during seasons of 2015/2016 and 2016/2017 towards maximizing its productivity under salt stress conditions. Pea seeds were inoculated with Rhizobium as a bio-fertilizer source before planting. Pea seeds were inoculated at sowing with N fixing bacteria (Rhizobium leguminosarum). The application of antioxidants to plants was done as foliar spraying after twenty days after planting and repeated three times a ten days interval. The following data were recorded, vegetative growth parameters, root nodule characteristics, leaves photosynthetic pigments content, fresh as well as dry yield characteristics and seed chemical compositions. Inoculation significantly increased all the studied traits of pea plant grown under salinity conditions compared with un-inoculation. Foliar spraying of pea plants with antioxidants also, significantly increased all the studied aspects of pea plant, SA+AA was more efficient than spraying either solely. Inoculation seeds with Rhizobium and foliar spray of SA+AA significantly increased all the studied characteristics. SA+AA along with inoculation was more effective in increasing all the studied characteristics. Inoculation with Rhizobium along with foliar spraying with SA+AA could be recommended for growing pea under saline conditions.
https://assjm.journals.ekb.eg/article_214706_096497443601f574d7850a0f502b2af1.pdf
pea
Rhizobium
antioxidants
Growth
yield and chemical compositions
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
591
604
10.21608/assjm.2018.214708
214708
Original Article
Effect of application of filter cake and biogas manures on improving of some desertic soil properties and their implications on plants grown thereon. I. Effect on chemical and physical properties
esmat Noufal
esmat.noufal@yahoo.com
1
The present investigation aims at studying the effect of application of biogas manure and filter cake (Filter mud), as a natural soil amendments, on improving some properties of desertic soils and their implications on barely plant grown thereon. A pot experiment was carried out at the greenhouse of soils and water department, Faculty of Agriculture, Benha University, Egypt, using perforated PVC pots of 5 kg capacity and barley (Hordum vulgare cv. Giza 123) as a test plant. Two surface soil samples (0 - 30 cm) differed in their texture and calcium carbonate content, were collected, to achieve these aims, from El-Hammam region, North-Western Coast of Egypt, to represent desertic soils. Biogas manure and filter cake (filter mud) were added at a rate of 0, 1, and 2 % (w/w) and thoroughly mixed with the soil and incubated for two months at moisture content equivalent to field capacity. Ten grains were planted in each pot. Irrigation was done with tap water so as to reach the water holding capacity of the soil. After complete germination plants were thinned to 5 per pot. Macronutrients of N, P and K were added to all pots according to the rates equivalent to those recommended by the Ministry of Agriculture. The experimental design was factorial randomized complete block design with two factors, in three replicates. At maturity, plants were harvested and soil samples were taken from each pot for physical and chemical analyses.
https://assjm.journals.ekb.eg/article_214708_4898fac8a0cde0c808c303daa57730e9.pdf
biogas manure
Filter cake
Calcareous soil
sandy soil
Physical properties
chemical properties
Barley plants
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
605
614
10.21608/assjm.2018.214709
214709
Original Article
Effect of applying filter cake and biogas manures on improving some desertic soil properties and their implications on plants grown thereon. II. Implications on plant growth
esmat Noufal
esmat.noufal@yahoo.com
1
The present investigation aims at studying the implications of applying biogas manure and filter cake (Filter mud) as a natural soil amendments on barely plant grown on desertic soils. Two surface soil samples (0 - 30 cm) differed in their texture and calcium carbonate content were collected from El-Hammam region, north-western coast of Egypt. A pot experiment was carried out at the greenhouse of Soils and Water Department, Faculty of Agriculture, Benha University, Egypt, using perforated PVC pots of 5 kg capacity. Biogas manure and filter cake (filter mud) were added at a rate of 0, 1, and 2 % (w/w) and thoroughly mixed with the soil and incubated for two months at moisture equivalent to field capacity. Barely plant (Hordum vulgare cv. Giza 123) was used as an indicator plant. Ten grains were planted in each pot. Irrigation was done using tap water so as to reach the water holding capacity of the soil. After complete germination plants were thinned to 5 per pot. Macronutrients; N, P and K were added to all pots according to the rates recommended by the Ministry of Agriculture. The experimental design was factorial randomized complete block design with two factors in three replicates. At maturity, the number of tillers and spikes and plant height were recorded. Plants were harvested and separated into grains and straw and analyzed for N, P and K. The obtained results show that:
The number of tillers and of spikes per pot significantly increased with increasing the rate of application of both amendments from R0 up to R2, whereas plant height (cm) significantly and progressively decreased with the rate. Filter cake was more efficient than biogas manure in this respect.
Application of biogas manure and filter cake significantly and progressively increased straw and grain yields in the sandy loam soil (S1), whereas significantly and progressively increased straw yield and decreased grain yield in the loamy sand soil (S2) and there was significant difference between biogas manure and filter cake in this respect. Application of biogas manure and filter cake significantly and progressively increased N, P and K uptake in barley straw with increasing the rate of application and there was significant difference between the two amendments in this respect. Nutrients uptake in barley grains significantly and progressively increased with increasing the rate of the applied amendment except for N uptake in grains of barley plants grown on the loamy sand soil (S2), where it decreased with increasing the application rate. In the case of the sandy loam soil (S1), biogas manure was more efficient than filter cake in increasing nutrients uptake in barley grains except for P uptake where filter cake was more efficient than biogas manure in this respect. In the case of the loamy sand soil (S2) biogas manure, also, was more efficient than filter cake in increasing P and K uptake in barley grains, whereas, both amendments decreased N uptake compared with R0 (non application of amendment) and the decrease was higher with the application of filter cake than with the application of the biogas manure. In conclusion the effect of the applied amendments on barley growth parameters, yield and nutrients uptake depends on the chemical properties and the rate of the applied amendment, and the soil characteristics developed upon the application of the organic amendment.
https://assjm.journals.ekb.eg/article_214709_e5ee23fb011788c51ef81cb1b0138557.pdf
biogas manure
Filter cake
Calcareous soil
sandy soil
Physical properties
chemical properties
Barley plants
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
615
622
10.21608/assjm.2018.214710
214710
Original Article
Efficient Use of N and Water for Maize (Zea Mays L.) Crop under Drip Irrigation System using 15N Stable Isotope
Ali Abdel-Salam
alyabsalam@yahoo.com
1
Soliman Soliman
solimanreh@yahoo.com
2
Soil and Water Department, Faculty of Agriculture, Moshtohor, Benha University, Egypt.
Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Abu-Zaable, Egypt
A field experiment was carried out on Maize (Zea Mays L.) grown on a sand soil during 2017 season to assess implications of N rates and irrigation water on yield and water consumption. The design was a factorial randomized complete block involving two factors: Factor W: 3 irrigation treatments as % of an Etc of 6588 m3 ha-1, i.e. 100% (W1), 80% (W2) and 70% (W3). Factor N: 3 N rates as % of a recommended 310 kg N ha-1,i.e.100% (N1) ,80% (N2) and 70% (N3). Grain yields (Mg ha-1) ranged from 2.80 (W1N3) to 4.48 (W2N2).Fertilizer N recovered in grains+stalks+leaves ranged from 5.4 % (W2N1) to 16.5% (W2N3). N use efficiency (kg grains kg-1 N) was 6.3 (W1N2) up to 18.6(W3N1). Water utilization efficiency (kg grains m-3 water) was 0.43 (W1 N3) to 0.84(W3 N3). High N and water use efficiency on sandy soils, must be by balanced combination water and N , and the general trend showed 5270 m3 water and 248 kg N per hectare (representing 80% of officially recommended inputs of each)
https://assjm.journals.ekb.eg/article_214710_1756f0a7c42736e75c0307b5d3a6ffba.pdf
NUE
WUE
recovery of fertilizer N in maize
15N-Isotope dilution
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
623
638
10.21608/assjm.2018.214714
214714
Original Article
Effect of location and fertilization on fruit yield and quality of some tomato cultivars
L.A.A Badr
lotfy.badr@fagr.bu.edu.eg
1
Horti.Dept., Fac., Benha Univ. , Moshtohor, Egypt.
Two open field experiments were performed during the two successive summer seasons of 2013and 2014 at El- Bosily,El-Behaira government and El-Fayuom, to investigate the growth and productivity of two tomato cultivars under different climatic conditions and different levels of NPK fertilizers. The experimental treatments were arranged by applying the split split plot design where, location treatments were arranged in the main plots, tomato cultivars treatments were arranged in the sub plots and fertilizer levels were arranged in the sub sub plots.Cultivated the tomato plants cv. Super Strain B in El Fayoum location combined with using 100% level from the recommended N.P.K doses exhibited the highest values of all fruit yield characteristics expressed as number of fruits/plant, fruit yield/ plant, total fruit yield/ fed., fruit/weight, length, diameter, shape and fruit set% compared with the other interaction treatments during the two seasons. On the other hand, using cv. Castel Rock and 80% from the recommended dose treatments in El Behaira location gave the lowest values. Meanwhile, cultivating the tomato plants in El Behaira by using cv. Super Strain B and 120 % from the recommended NPK doses exhibited the highest values of all of chemical fruit quality of tomato plant (Nitrogen%, Phosphorus%, Potassium%, total carbohydrates, T.S.S and total acidity, compared with the other interaction treatments during the two seasons. On the contrary, using Cv. Castle Rock and 80% from the recommended NPK dose in El Fayoum location gave the lowest values.
https://assjm.journals.ekb.eg/article_214714_8d7e91b20ff0eb6c55a2e26018e31e1b.pdf
Location
NPK fertilizers
Fruit yield
Quality
Tomato cultivars
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
639
644
10.21608/assjm.2018.214716
214716
Original Article
Wheat Lodging and Yield in Response to Cultivars and Foliar Application of Paclobutrazol
Two field experiments were conducted at the Research and Experimental Center of Faculty of Agriculture at Moshtohor, Benha University, Egypt, during 2015/16 and 2016/17 winter seasons to evaluate five wheat cultivars includes Misr 2, Gemmeza 11, Shandaweel 1, Giza 171 and Sids 12 under threefoliar application of Paclobutrazol }plant growth retardant (PGR){ at rates 0, 100, 200 ppm fad-1 for root lodging, yield and yield components. The obtained results for combined analyses were as follows:
The significant effect of PGR observed for root dry weight plant-1, plant height, No. of tillers m-2, No. of spikes m-2, No. of kernels spike-1 and straw yield fed-1. Cultivars were varied significantly in lodging score, root dry weight plant-1, plant height, No. of tillers m-2, No. of spikes m-2, seed index, grain yield fed-1, straw yield fed-1 and harvest index. While PGR×cultivars interactions showed no significance effect on all these traits.
https://assjm.journals.ekb.eg/article_214716_92bc07012e52bd8a5e64e810008174ae.pdf
Spring wheat
Foliar application of Paclobutrazol
cultivars
yield and its components
eng
Benha University; Faculty of Agriculture
Annals of Agricultural Science, Moshtohor
1110-0419
2974-4830
2018-04-01
56
4th ICBAA
645
656
10.21608/assjm.2018.214717
214717
Original Article
Effect of some safety compounds and application method on growth and productivity of globe artichoke (Cynara scolymus, l.)
A. Shams
abdelhakeem.shams@fagr.bu.edu.eg
1
Horticulture Dept., Faculty of Agriculture, Benha University, Benha, Egypt.
Two field experiments were conducted during two successive seasons of 2014/2015 and 2015/2016 at Vegetable Experimental Research Farm in Kaha, Kaliobia Governorate to study the effect of application method of safety compounds on growth and productivity of globe artichoke cv. "Concerto” used treatments included three different application method; i.e., soaking 20 min., spraying 6 times started after 70 days from planting date and repeated every 15 days through the growth season and soaking plus spraying in seven different safety compounds; i.e., Amino power (source of free amino acids) 0.5 cm3/l, Fruiting (source of gibberellins) 0.5 cm3/l, Mega humic acid (source of humic acid) 0.25 g/l, Cytojeep (source of gibberellins plus free amino acids) 0.5 cm3/l, Chitosan 1% and Garlic extract 50 cm3/l as well as tap water (control). A split plot design with four replicates was used in this experiment. Main plots were application method and sub-plots were safety compounds.
The obtained results revealed that spraying Mega humic acid or soaking plus spraying significantly produced the highest leaves number/ plant, plant height, produced yield (early, late and total) expressed as heads weight / plant, head and edible part weight (early and late yield), dry matter percentage in late yield as well as the lowest total phenols in early yield comparing with the control. On the other hand, using Cytojeep significantly produced the highest offshoots number / plant and the least number of days to the anthesis. Also, spraying Fruiting significantly increased inuline percentage in early and late yield and decreased the fibers contents in late yield.
https://assjm.journals.ekb.eg/article_214717_8bc10e82ebc2a4d91febe08430c63f3b.pdf
Cynara scolymus L
Humic acid
garlic extract
Chitosan
gibberellins
Soaking
spraying